A PRECURSOR PRODUCT RELATIONSHIP EXISTS BETWEEN OVAL CELLS AND HEPATOCYTES IN RAT-LIVER
A PRECURSOR PRODUCT RELATIONSHIP EXISTS BETWEEN OVAL CELLS AND HEPATOCYTES IN RAT-LIVER
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DOI：
10.1093/carcin/8.11.1737
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发表时间：
1987-11-01
期刊：
影响因子：
4.7
通讯作者：
THORGEIRSSON, SS
中科院分区：
文献类型：
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作者：
EVARTS, RP;NAGY, P;THORGEIRSSON, SS
12只雄性Fischer大鼠分别给予2-乙酰氨基芴（2- aaf） 2周和给予2- aaf 1周后行部分肝切除术诱导卵圆细胞增殖。用3h标记的大鼠白蛋白核糖探针原位杂交法研究了肝脏白蛋白的表达。在部分肝切除术后第6天给予一组动物放射标记胸苷。分别于肝部分切除后0、3、7、9、11、13天处死。卵形细胞和嗜碱性肝细胞均显著表达白蛋白，而嗜酸性肝细胞中白蛋白表达降低。[3H]胸苷嘧啶给药1天后，卵圆细胞核被单独标记。在第9、11和13天，嗜碱性肝细胞被标记，这些细胞占据的面积增加。这是第一次证明放射性标记胸苷从卵形细胞转移到体内新形成的肝细胞。因此，卵形细胞与嗜碱性肝细胞之间的前体-产物关系已经建立。
Oval cell proliferation was induced in twelve male Fischer rats by administration of 2-acetylaminofluorene (2-AAF) for 2 weeks and by performing partial hepatectomy one week after the beginning of 2-AAF administration. Albumin expression in liver was studied by using in situ hybridization of 3H-labeled rat albumin riboprobe. Radiolabeled thymidine was administered to a group of animals at day 6 after partial hepatectomy. The animals were killed at 0, 3, 7, 9, 11 and 13 days after partial hepatectomy. Both oval cells and basophilic hepatocytes showed a prominent expression of albumin, whereas albumin expression in acidophilic preexisting hepatocytes was decreased. Oval cell nuclei were exclusively labeled one day after administration of [3H]thymidine. At day 9, 11 and 13 basophilic hepatocytes became labeled and the area occupied by these cells increased. This is the first demonstration of the transfer of radiolabeled thymidine from oval cells to newly formed hepatocytes in vivo. Thus the precursor-product relationship between oval cells and basophilic hepatocytes has been established.