Functional motor recovery after peripheral nerve repair with an aligned nanofiber tubular conduit in a rat model
Functional motor recovery after peripheral nerve repair with an aligned nanofiber tubular conduit in a rat model
复制标题
DOI：
10.2217/rme.12.87
复制
发表时间：
2012-11-01
影响因子：
2.7
通讯作者：
Kuo, Alfred C.
中科院分区：
文献类型：
--
作者：
Jin, Jenny;Park, Michelle;Kuo, Alfred C.
目的：目前合成管状导管在修复节段性周围神经损伤方面不如自体神经移植物。我们在大鼠模型中研究了使用纵向排列的聚（L-丙交酯-己内酯）血管导管修复神经间隙损伤的运动结果。方法：44只刘易斯大鼠，切断坐骨神经10 mm段。缺损未修复6例，自体神经移植修复19例，导管修复19例。12周后测定神经传导潜伏期、复合肌肉动作电位振幅、肌力和肌肉质量。测定移植物内和远端的轴突数量和轴突直径。结果如下：12周后，管道组腓肠肌等长强直力和肌肉质量分别达到自体移植值的85%和82%。神经传导和复合肌肉动作电位在这两组之间没有显著差异，尽管后者接近显著性。未修复组无恢复。结论：用这种对齐的神经导管治疗的动物的肌肉恢复接近自体移植物，表明内部导管结构对神经修复的重要性。
Aim: Current synthetic tubular conduits are inferior to nerve autograft for the repair of segmental peripheral nerve injuries. We examined motor outcomes with the use of longitudinally aligned poly (L-lactide-co-caprolactone) nanofiber conduits for repair of nerve gap injury in a rat model. Methods: Ten-millimeter segments of sciatic nerve were resected in 44 Lewis rats. The gaps were either left unrepaired (n = 6), repaired with nerve autograft (n = 19), or repaired with conduit (n = 19). After 12 weeks, nerve conduction latency, compound muscle action potential amplitude, muscle force and muscle mass were measured. The numbers of axons and axon diameters both within the grafts and distally were determined. Results: After 12 weeks, gastrocnemius isometric tetanic force and muscle mass for the conduit group reached 85 and 82% of autograft values, respectively. Nerve conduction and compound muscle action potential were not significantly different between these two groups, although the latter approached significance. There was no recovery in the unrepaired group. Conclusion: Muscle recovery for the animals treated with this aligned nanofiber conduit approached that of autograft, suggesting the importance of internal conduit structure for nerve repair.