Molecular analysis of the interaction between Olea europaea and the biotrophic fungus Spilocaea oleagina
Molecular analysis of the interaction between Olea europaea and the biotrophic fungus Spilocaea oleagina
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DOI：
10.1111/j.1364-3703.2005.00290.x
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Muñoz-Blanco, J
中科院分区：
文献类型：
--
作者：
Benitez, Y;Botella, MA;Muñoz-Blanco, J
油橄榄溢尾藻是一种专性生体营养型真菌，它能引起橄榄叶黑星病，导致橄榄叶脱落和产量损失。利用差异显示技术，我们鉴定了162个cDNA片段，这些片段对应于在抗S.橄榄油通过实时定量RT-PCR对21个选定的基因进行详细分析，揭示了不同的诱导动力学。参与信号传导、转录调控、氧化胁迫、生物和非生物胁迫的基因以及一些功能未知的基因在感染后被迅速诱导。相反，参与代谢和细胞维持的基因表现出延迟诱导。在感病品种中，选择基因的诱导在对S.橄榄油有趣的是，一些基因的基础表达在未感染的抗品种高于感病品种，这表明防御反应的组成性激活。这些基因的表达响应水杨酸，茉莉酸甲酯，两者的混合物，乙烯利，过氧化氢，甲萘醌和创伤也进行了研究。结果进行了讨论，在有关的分子基础和信号事件参与这种生物营养的相互作用。
The mitosporic fungus Spilocaea oleagina is an obligate biotroph of olive (Olea europaea) causing a scab disease associated with leaf fall and substantial losses in production. Using differential display we have identified 162 cDNA fragments corresponding to transcripts that show altered abundance during the defence response of a resistant olive cultivar to S. oleagina. Detailed analyses of 21 selected genes by real-time quantitative RT-PCR revealed different kinetics of induction. Genes involved in signalling, transcriptional control, oxidative stress, biotic and abiotic stress, and several genes with unknown function were found to be induced rapidly after infection. In contrast, genes involved in metabolism and cellular maintenance showed delayed induction. The induction of the selected genes in a susceptible cultivar was delayed and/or reduced during the response to S. oleagina. Interestingly, the basal expression of some genes in the uninfected resistant cultivar was higher than in the susceptible one, suggesting a constitutive activation of defence responses. Expression of these genes in response to salicylic acid, methyl jasmonate, a mixture of both, ethephon, hydrogen peroxide, menadione and wounding was also investigated. The results are discussed in relation to the molecular bases and signalling events involved in this biotrophic interaction.