Trimethylamine and trimethylamine N-oxide are supplementary energy sources for a marine heterotrophic bacterium: implications for marine carbon and nitrogen cycling
Trimethylamine and trimethylamine N-oxide are supplementary energy sources for a marine heterotrophic bacterium: implications for marine carbon and nitrogen cycling
复制标题
DOI：
10.1038/ismej.2014.149
复制
发表时间：
2015-03-01
期刊：
影响因子：
11
通讯作者：
Chen, Yin
中科院分区：
文献类型：
--
作者：
Lidbury, Ian D. E. A.;Murrell, J. Colin;Chen, Yin
海洋蔷薇科细菌的特点是它们能够利用各种有机和无机化合物来支持生长。三甲基胺（TMA）和氧化三甲基胺（TMAO）是甲基化胺（MA），是海洋中溶解有机氮库的一部分，是仅次于N-2气体的第二大氮源。我们调查了海洋异养细菌，Ruegeria pomeroyi DSS-3，是否可以利用TMA和TMAO作为补充能源，以及这种特性是否对生长有任何有益的影响。In R. pomeroyi、TMA和TMAO的催化作用导致细胞内ATP的产生，这反过来有助于提高生长速率和生长产量，以及在长时间的能量饥饿期间提高细胞存活。此外，同时使用两种不同的外源能源导致了更大的增强化能异养生长。使用TMA和TMAO主要作为能源导致氮以铵的形式被矿化，这可以交叉喂养到另一种细菌中。这项研究提供了更深入的了解微生物代谢的MA在海洋环境中，以及它如何可能会影响海洋表面沃茨内的营养流和这些气候重要的化合物进入大气中的通量。
Bacteria of the marine Roseobacter clade are characterised by their ability to utilise a wide range of organic and inorganic compounds to support growth. Trimethylamine (TMA) and trimethylamine N-oxide (TMAO) are methylated amines (MA) and form part of the dissolved organic nitrogen pool, the second largest source of nitrogen after N-2 gas, in the oceans. We investigated if the marine heterotrophic bacterium, Ruegeria pomeroyi DSS-3, could utilise TMA and TMAO as a supplementary energy source and whether this trait had any beneficial effect on growth. In R. pomeroyi, catabolism of TMA and TMAO resulted in the production of intracellular ATP which in turn helped to enhance growth rate and growth yield as well as enhancing cell survival during prolonged energy starvation. Furthermore, the simultaneous use of two different exogenous energy sources led to a greater enhancement of chemoorganoheterotrophic growth. The use of TMA and TMAO primarily as an energy source resulted in the remineralisation of nitrogen in the form of ammonium, which could cross feed into another bacterium. This study provides greater insight into the microbial metabolism of MAs in the marine environment and how it may affect both nutrient flow within marine surface waters and the flux of these climatically important compounds into the atmosphere.