Discovering the antibacterial mode of action of 3-p-trans-coumaroyl-2-hydroxyquinic acid, a natural phenolic compound, against Staphylococcus aureus through an integrated transcriptomic and proteomic approach
Discovering the antibacterial mode of action of 3-p-trans-coumaroyl-2-hydroxyquinic acid, a natural phenolic compound, against Staphylococcus aureus through an integrated transcriptomic and proteomic approach
复制标题
通过综合转录组学和蛋白质组学方法发现 3-p-反式香豆酰基-2-羟基奎尼酸（一种天然酚类化合物）针对金黄色葡萄球菌的抗菌作用模式
DOI：
10.1111/jfs.12861
复制
发表时间：
2020
影响因子：
2.4
通讯作者：
Hong Gao
中科院分区：
文献类型：
--
作者：
Xiaoyan Liu;Yuxi Yue;Yanping Wu;Kai Zhong;Qian Bu;Hong Gao
最近报道了一种植物来源的酚类化合物，即3-对-反-香豆酰基-2-羟基奎尼酸（CHQA），对金黄色葡萄球菌表现出抗菌和抗菌膜活性。在这项研究中，结合转录组学和蛋白质组学分析被用来阐明CHQA对金黄色葡萄球菌的分子机制。结果表明，亚抑制浓度的CHQA诱导金黄色葡萄球菌在转录和翻译水平上发生了广泛而显著的变化，共产生935个差异表达基因和438个差异表达蛋白。生物信息学分析表明，这些基因和蛋白质的变化主要涉及细胞膜、核糖体和翻译、DNA修复、脂肪酸的合成和代谢、氨基酸的合成、肽聚糖的合成以及细菌感染等方面。此外，下调与细胞粘附的各种表面蛋白的观察，这可能进一步抑制生物膜形成ofs. aureus。这些结果表明，CHQA通过多种机制发挥抗菌活性，特别是针对细菌细胞膜，然后引发各种细胞功能障碍。
A plant‐derived phenolic compound, namely 3‐p‐trans‐coumaroyl‐2‐hydroxyquinic acid (CHQA), has recently been reported to exhibit antibacterial and antibiofilm activities againstStaphylococcus aureus. In this study, the combined transcriptomic and proteomic analyses was used to elucidate the molecular mechanism of CHQA againstS.aureus. The results showed that subinhibitory concentration of CHQA induced wide and significant changes inS.aureusat both transcriptional and translational levels with 935 differentially expressed genes and 438 differentially expressed proteins. Bioinformatic analysis indicated that the changed genes and proteins were mainly involved in cell membrane, ribosome and translation, DNA repair, fatty acid biosynthesis and metabolism, amino acid biosynthesis, peptidoglycan synthesis, and bacterial infection. Moreover, downregulation of various surface proteins associated with cell adhesion was observed, which probably further inhibited the biofilm formation ofS.aureus. These results revealed that CHQA exerted antibacterial activity through multifarious mechanisms, which especially targeted bacterial cell membrane and then triggered diverse cellular dysfunctions.