A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.
A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.
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与哺乳动物转录因子 IIA 相关的 TATA 序列依赖性转录抑制活性。
DOI：
10.1002/j.1460-2075.1994.tb06278.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Conaway,JW
中科院分区：
文献类型：
--
作者：
Aso,T;Serizawa,H;Conaway,RC;Conaway,JW
在鉴定由RNA聚合酶II调控基础转录的细胞蛋白的过程中，我们鉴定了一种新的针对含有共识TATA盒的启动子的阻遏活性。该活性强烈抑制来自含有一致TATA序列的核心启动子元件的TBP依赖的转录启动，但激活来自缺乏一致TATA序列的核心启动子元件的TBP依赖的转录。将这种活性从大鼠肝核提取物中纯化到近乎均一的水平，导致了令人惊讶的发现，它与哺乳动物转录因子IIA(TFIIA)密切协同纯化。TATA序列依赖的转录抑制因子活性与TFIIA的密切联系为该因子在RNA聚合酶II转录中的复杂功能增加了一个新的和意想不到的维度。
In the process of characterizing cellular proteins that modulate basal transcription by RNA polymerase II, we identified a novel repressor activity specific for promoters containing consensus TATA boxes. This activity strongly represses TATA‐binding protein (TBP)‐dependent transcription initiation from core promoter elements containing a consensus TATA sequence, but activates TBP‐dependent transcription from core promoter elements lacking a consensus TATA sequence. Purification of this activity to near homogeneity from rat liver nuclear extracts led to the surprising discovery that it co‐purifies closely with mammalian transcription factor IIA (TFIIA). The close association of TATA sequence‐dependent transcriptional repressor activity with TFIIA adds a new and unexpected dimension to the already complex picture of this factor's function in transcription by RNA polymerase II.