THE CONCENTRATION OF RETINOIC ACID DETERMINES THE DIFFERENTIATED CELL-TYPES FORMED BY A TERATOCARCINOMA CELL-LINE
THE CONCENTRATION OF RETINOIC ACID DETERMINES THE DIFFERENTIATED CELL-TYPES FORMED BY A TERATOCARCINOMA CELL-LINE
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DOI：
10.1016/0012-1606(83)90348-2
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
MCBURNEY, MW
中科院分区：
文献类型：
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作者：
EDWARDS, MKS;MCBURNEY, MW
视黄酸（RA）可诱导体外培养的畸胎瘤细胞分化。对于[小鼠]畸胎瘤细胞系P19 S1801 A1（O 1A 1），由暴露于RA的细胞聚集体形成的分化细胞类型取决于治疗中使用的药物浓度。暴露于低浓度（10-9 M）的培养物的特征是心肌丰富。骨骼肌在较高药物浓度（10-8 M）下变得丰富，神经元和星形胶质细胞在极高浓度（10-7 - 10-5 M）下出现。显然，在正常胚胎发生过程中，多能细胞对特定发育途径的承诺可能部分取决于物质（如类维生素A）的浓度梯度。
Retinoic acid (RA) can induce the differentiation of teratocarcinoma cells in culture. With the [mouse] teratocarcinoma cell line, P19S1801A1 (O1A1), the differentiated cell types formed from cell aggregates exposed to RA are dependent on the concentration of drug used in the treatment. Cultures exposed to low concentrations (10-9 M) are characterized by an abundance of cardiac muscle. Skeletal muscle becomes abundant at higher drug concentrations (10-8 M) with neurons and astroglia appearing at very high concentrations (10-7 - 10-5 M). Evidently, during normal embryogenesis, the commitment of pluripotent cells to particular developmental avenues may be determined in part by concentration gradients of substances such as retinoids.