Analysis of the cytogenetic stability of the human embryonal kidney cell line 293 by cytogenetic and STR profiling approaches
Analysis of the cytogenetic stability of the human embryonal kidney cell line 293 by cytogenetic and STR profiling approaches
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DOI：
10.1159/000078556
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发表时间：
2004-01-01
影响因子：
1.7
通讯作者：
Weber, G
中科院分区：
文献类型：
--
作者：
Bylund, L;Kytölä, S;Weber, G
我们用光谱核型分析和G显带分析研究了人胚胎细胞系293的细胞遗传学改变。为了研究其基因组稳定性，我们比较了293及其子系ECR-293的核型。通过短串联重复序列进行的基因分型补充了这项分析。虽然这两个品系的STR图谱几乎相同，从而证实了它们的起源和亲缘关系，但它们的染色体数量和异常染色体的设置明显不同。然而，在长期培养中，该细胞系保持了稳定的核型。ECR-293亚克隆的建立，表达了可诱导的LacZ或MEN1转基因，只增加了核型的微小变化。我们的研究表明，起源于293的克隆细胞系的细胞遗传学构成似乎足够稳定。然而，在比较独立的293个谱系的特性时要小心，因为克隆变异可能是很大的。版权所有(C)2004 S.Karger AG，巴塞尔。
We have characterized the cytogenetic alterations of the human embyronal cell line 293 by spectral karyotyping and G-banding analysis. To investigate its genomic stability, we compared the karyotypes of 293 and its daughter line EcR-293. Genotype profiling through short tandem repeats complemented the analysis. While displaying almost identical STR profiles and thus verifying their origin and their close relation, the two lines were remarkably different in their number of chromosomes and setup of aberrant chromosomes. However, the cell lines retained a stable karyotype in long term culture. The establishment of subclones from EcR-293, expressing inducible lacZ or MEN1 transgenes, only added minor changes to the karyotype. Our study shows that the cytogenetic constitution of a clonal cell line of the 293 origin appears to be sufficiently stable. However, care should be taken when comparing the properties of independent 293 lineages, since clonal variations might be substantial. Copyright (C) 2004 S. Karger AG, Basel.