In vitro and in vivo differentiation of human umbilical cord derived stem cells into endothelial cells
In vitro and in vivo differentiation of human umbilical cord derived stem cells into endothelial cells
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DOI：
10.1002/jcb.21078
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发表时间：
2007-02-15
影响因子：
4
通讯作者：
Liu, Ying Long
中科院分区：
文献类型：
--
作者：
Wu, Kai Hong;Zhou, Bin;Liu, Ying Long
组织工程移植的成功应用受到血管化需要的阻碍。最近的进展已经使得使用干细胞作为治疗性血管生成的细胞来源成为可能，包括工程化组织移植物的血管化。本研究的目的是检测人脐带源性干细胞（UCDS）的内皮潜能。UCDS细胞最初的特点和分化的内皮细胞分化培养基中含有VEGF和bFGF。通过乙酰化低密度脂蛋白掺入和内皮特异性蛋白（如PECAM和CD34）的表达确定向内皮细胞的分化。在体内，移植的UCDS细胞从局部注射发芽，并在后肢缺血模型中分化为内皮细胞。这些发现表明在人脐带中存在具有内皮祖细胞特征的细胞群。因此，人脐带可能是一种干细胞来源，可用于治疗性血管生成和工程组织移植物的再内皮化。
The successful use of tissue-engineered transplants is hampered by the need for vascularization. Recent advances have made possible the using of stem cells as cell sources for therapeutic angiogenesis, including the vascularization of engineered tissue grafts. The goal of this study was to examine the endothelial potential of human umbilical cord-derived stem (UCDS) cells. UCDS cells were initially characterized and differentiated in an endothelial differentiation medium containing VEGF and bFGF. Differentiation into endothelial cells was determined by acetylated low-density lipoprotein incorporation and expression of endothelial-specific proteins, such as PECAM and CD34. In vivo, the transplanted UCDS cells were sprouting from local injection and differentiated into endothelial cells in a hindlimb ischemia mouse model. These findings indicate the presence of a cell population within the human umbilical cord that exhibits characteristics of endothelial progenitor cells. Therefore, human umbilical cord might represent a source of stem cells useful for therapeutic angiogenesis and re-endothelialization of engineered tissue grafts.