Toehold-regulated competitive assembly to accelerate the kinetics of graphene oxide-based biosensors
Toehold-regulated competitive assembly to accelerate the kinetics of graphene oxide-based biosensors
复制标题
立足点调节竞争组装加速基于氧化石墨烯的生物传感器的动力学
DOI：
10.1039/c9tb02454a
复制
发表时间：
2020
影响因子：
7
通讯作者：
Cheng Nansheng
中科院分区：
文献类型：
--
作者：
Du Huan;Chen Junbo;Zhang Jie;Zhou Rongxing;Yang Peng;Hou Xi;eng;Cheng Nansheng
With effective adsorption and quenching efficiency, graphene oxide (GO) can be utilized for sensing biomolecules such as nucleic acids and proteins. In these assays, the fluorophore-labeled nucleic acid (reporter) is usually adsorbed by GO first, followed by adding the target molecules to bind the reporter, thus restoring the fluorescence signal. However, the kinetics of fluorescence recovery is usually very slow because the target is probably adsorbed by GO and compromises the binding of the target and the reporter. Herein, we proposed a toehold-regulated strand displacement strategy to accelerate the kinetics of GO-based biosensing. In this strategy, the toehold of the duplex mediated a competitive assembly with the aim of eliminating the adsorption of the target by GO, facilitating the binding of the target and the reporter. While the duplex with the toehold of the target-blocker DNA or reporter-blocker DNA was formed, the rigid structure of the duplex weakened the adsorption of the target by GO and enhanced the recognition of the target by the reporter. This strategy achieved up to 2.6-fold enhancement in fluorescence signal restoration for nucleic acid detection, while there was 3.2-fold enhancement in fluorescence signal restoration for thrombin detection. It has also been demonstrated that this strategy can be used for the determination of DNA and thrombin in diluted serum with excellent specificity.
