The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family
The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family
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DOI：
10.1073/pnas.93.10.4804
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发表时间：
1996-05-14
影响因子：
11.1
通讯作者：
Bohlander, SK
中科院分区：
文献类型：
--
作者：
Dreyling, MH;MartinezCliment, JA;Bohlander, SK
t(10;11)(p13;q14)易位是一种反复出现的染色体异常，在急性淋巴细胞白血病以及急性髓系白血病患者中均有发现。我们最近报道单核细胞系U937存在t(10;11)(p13;q14)易位。通过定位克隆和候选基因方法相结合，我们克隆了断点，并能够证明AF10与一个我们命名为CALM（网格蛋白装配淋巴细胞髓系白血病基因）的新基因融合，该基因位于11q14。AF10是一种假定的转录因子，最近作为MLL的融合伴侣之一被克隆。CALM在其N末端三分之一处与小鼠ap - 3基因具有非常高的同源性，ap - 3基因是网格蛋白装配蛋白之一。已表明ap - 3的N末端区域与网格蛋白结合，并具有磷酸肌醇的高亲和力结合位点。在广泛使用的U937细胞系中CALM/AF10融合基因的鉴定将有助于我们理解该细胞系的恶性表型。
The translocation t(10:11)(p13;q14) is a recurring chromosomal abnormality that has been observed in patients with acute lymphoblastic leukemia as well as acute myeloid leukemia. We have recently reported that the monocytic cell line U937 has a t(10;11)p13;q14) translocation. Using a combination of positional cloning and candidate gene approach, we cloned the breakpoint and were able to show that AF10 is fused to a novel gene that we named CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene) located at 11q14. AF10, a putative transcription factor, had recently been cloned as one of the fusion partners of MLL. CALM has a very high homology in its N-terminal third to the murine ap-3 gene which is one of the clathrin assembly proteins. The N-terminal region of ap-3 has been shown to bind to clathrin and to have a high-affinity binding site for phosphoinositols. The identification of the CALM/AF10 fusion gene in the widely used U937 cell line will contribute to our understanding of the malignant phenotype of this line.