Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes.
Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes.
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通过使用金纳米棒探针的单分子显微镜观察到大量的大肠杆菌 F1-ATPase 分子旋转。
DOI：
10.1007/s10863-007-9114-x
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发表时间：
2007
影响因子：
3
通讯作者：
Frasch,WayneD
中科院分区：
文献类型：
--
作者：
York,Justin;Spetzler,David;Hornung,Tassilo;Ishmukhametov,Robert;Martin,James;Frasch,WayneD
E.对观察到的使用连接到γ-亚基的金纳米棒旋转的coliF 1-ATPase分子进行定量。当通过偏振滤光器观察时，基于从纳米棒散射的红色和绿色光的时间依赖性波动，确定单个F1分子是旋转的。在有GTP、ATP和无核苷酸的情况下，观察到的F1分子的平均旋转数分别为1.50%、1.25%和1.4%。在一些实验中，观察到在GTP存在下旋转的分子的分数高达65%。这些数据表明，使用金纳米棒进行的旋转测量提供了F1-ATP酶机制的信息，该机制代表了整个酶群体的特征。
The abundance ofE. coliF1-ATPase molecules observed to rotate using gold nanorods attached to the γ-subunit was quantitated. Individual F1molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was ∼50, ∼25, and ∼4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole.