A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
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DOI：
10.1089/zeb.2016.1385
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发表时间：
2017-06
期刊：
影响因子：
2
通讯作者：
Harris WA
中科院分区：
文献类型：
--
作者：
MacDonald RB;Kashikar ND;Lagnado L;Harris WA
谷氨酸是大脑中主要的兴奋性神经递质。它的释放和最终的回收是快速持续神经活动的关键。我们已经将gfap启动子区与谷氨酸报告分子iGluSnFR配对，以驱动整个神经系统的神经胶质细胞中的表达。Tg（gfap：iGluSnFR）在视网膜中的Müller神经胶质细胞的神经胶质膜上表达，其快速响应刺激和细胞外谷氨酸的释放。由于神经胶质细胞与大多数（如果不是全部）突触相关，因此Tg（gfap：iGluSnFR）是一种新颖且令人兴奋的工具，可用于测量神经系统许多区域的神经元活动和细胞外谷氨酸动力学。
Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfap:iGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.