Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples.
Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples.
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通过颈部皮肤样本的聚合酶链反应诊断人类狂犬病病例。
DOI：
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发表时间：
2006
影响因子：
3.4
通讯作者：
I. Kotait
中科院分区：
文献类型：
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作者：
C. Macedo;P. Carnieli;P. Brandão;E. S. Travassos da Rosa;R. Oliveira;J. Castilho;R. Medeiros;R. Machado;Rosely Cerqueira de Oliveira;M. Carrieri;I. Kotait
对疑似人类病例的狂犬病快速诊断影响了对患者潜在接触者的暴露后预防，并确保了适当的患者管理。除了中枢神经系统（CNS），狂犬病病毒（RABV）通常存在于受感染人类毛囊附近的小感觉神经中。我们使用RT-PCR，引物针对核蛋白基因（N）的3'末端部分，测试9例狂犬病患者的颈部皮肤样本，以验证一种诊断方法，可作为狂犬病诊断的额外工具，特别是在死前样本中。用RT-PCR方法检测8份尸体标本中有6份为狂犬病阳性，2份尸体标本中有1份为狂犬病阳性。DNA测序证实了结果，这验证了RT-PCR和颈部皮肤作为一个合适的技术和类型的样本，分别用于诊断人狂犬病。RT-PCR应用于颈部皮肤活检可以早期诊断，并导致更有效的狂犬病治疗。
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment.