cDNA cloning and gene characterization of the mitochondrial large subunit (LSU) rRNA from the liver fluke Fasciola hepatica. Evidence of heterogeneity in the fluke mitochondrial genome.
cDNA cloning and gene characterization of the mitochondrial large subunit (LSU) rRNA from the liver fluke Fasciola hepatica. Evidence of heterogeneity in the fluke mitochondrial genome.
复制标题
肝片形吸虫线粒体大亚基 (LSU) rRNA 的 cDNA 克隆和基因表征。
DOI：
10.1093/nar/16.14.7001
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发表时间：
1988
影响因子：
14.9
通讯作者：
Mansour,TE
中科院分区：
文献类型：
--
作者：
Zurita,M;Bieber,D;Ringold,G;Mansour,TE
从肝吸虫（F.hepatica）的肝组织中分离并鉴定了一个编码线粒体核糖体RNA大亚基（LSU rRNA）的cDNA克隆。该RNA分子在3 ′端被多聚腺苷酸化，占成体肝片吸虫中多聚A + RNA的10%。Fluke LSU rRNA与蚊的线粒体LSU rRNA具有显著的序列同源性，并且与雌雄异株吸虫的线粒体rRNA的关系比雌雄异株吸虫的更为密切。线粒体DNA约占成虫细胞DNA的10%。这个百分比在无胚蛋中较低，LSU rRNA水平也较低，表明蛋的代谢活性较低。对曼氏血吸虫线粒体基因组的转录和数量的分析表明，LSU rRNA在雌性比雄性更丰富。吸虫线粒体LSU rRNA基因的限制性内切酶分析表明，在线粒体基因组中存在异质性重复拷贝或线粒体的个体基因组之间的异质性。
A cDNA clone that encodes the large subunit of mitochondrial ribosomal RNA (LSU rRNA) from the liver flukeF.hepaticawas isolated and characterized. This RNA molecule is polyadenylated at the 3′ end and represents 10% of the poly A+RNA in adultF.hepatica. Fluke LSU rRNA has significant sequence homology to mosquito mitochondria LSU rRNA and is more closely related to the mitochondrial rRNA of hermaphroditic than dioecious trematodes. Mitochondrial DNA constitutes approximately 10% of the total cellular DNA of adult flukes. This percentage is lower in non-embryonated eggs as are the levels of LSU rRNA indicating eggs have lower metabolic activity. Analysis of transcription and the number of mitochondrial genomes inS.mansonishows that the LSU rRNA is more abundant in females than in males. Restriction endonuclease analysis of the fluke mitochondrial LSU rRNA genes suggests the presence of heterogeneous repeated copies in the mitochondrial genome or heterogeneity among individual genomes of mitochondria.
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DOI：
10.1016/s0002-9343(84)80006-6
发表时间：
1984
期刊：
The American journal of medicine
影响因子：
--
作者：
Morgan,JP;Morgan,KG
通讯作者：
Morgan,KG
影响因子：
37.8
作者：
M. Endoh;T. Yanagisawa;N. Taira;Blinks
通讯作者：
M. Endoh;T. Yanagisawa;N. Taira;Blinks
米力农、多巴酚丁胺和硝普钠：对严重充血性心力衰竭患者血流动力学和心肌能量学的比较影响。
影响因子：
37.8
作者：
Monrad,ES;Baim,DS;Smith,HS;Lanoue,AS
通讯作者：
Lanoue,AS
异丙肾上腺素、胰高血糖素和乙酰胆碱对心肌环 AMP 的调节。
DOI：
--
发表时间：
1971
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
T. P. Lee;J. Kuo;P. Greengard
通讯作者：
P. Greengard
MCI-154（一种新型强心剂）在豚鼠乳头肌的化学剥皮纤维中增加收缩系统的 Ca 敏感性。
影响因子：
3.5
作者：
Y. Kitada;A. Narimatsu;N. Matsumura;M. Endo
通讯作者：
M. Endo