Mitochondrial bioenergetics as affected by cationic detergents
Mitochondrial bioenergetics as affected by cationic detergents
复制标题
阳离子洗涤剂对线粒体生物能的影响
DOI：
10.1007/bf00215809
复制
发表时间：
1996
影响因子：
4
通讯作者：
P. Dell'Antone
中科院分区：
文献类型：
--
作者：
M. Bragadin;P. Dell'Antone
本文研究了阳离子去污剂十六烷基三甲基溴化铵（CTAB）在通电大鼠肝线粒体中的积累和毒性机制。结果表明：（1）CTAB离子通过膜电位驱动的摄取机制在线粒体基质中蓄积;（2）蓄积可能导致毒性效应，因为它引起线粒体膜电位（ΔΦ）的崩溃，该电位与ATP合成相关并调节Ca++摄取;（3）ΔΦ的崩溃可能是由于线粒体膜对离子的渗透性增强（4）在另一种阳离子洗涤剂NTAB中也观察到ΔΦ塌陷和Ca++、K+释放，但在阴离子洗涤剂存在下没有观察到。
This paper examines the accumulation and toxicity mechanism of a cationic detergent, cetyltrimethylammonium (bromide) (CTAB), in energized rat liver mitochondria. The results suggest that: (1) the CTAB ion is accumulated in the mitochondrial matrix by a membrane potential-driven uptake mechanism; (2) accumulation may lead to a toxic effect, since it gives rise to collapse of the mitochondrial membrane potential (ΔΦ) which is correlated with ATP synthesis and which regulates Ca++uptake; (3) collapse of ΔΦ may be due to enhanced permeability of the mitochondrial membrane to the ions (detergent effect); and (4) ΔΦ collapse and Ca++and K+release were also observed in another cationic detergent, NTAB, but not in the presence of anionic detergents.