A transcript map of the chromosome 19q-arm glioma tumor suppressor region
A transcript map of the chromosome 19q-arm glioma tumor suppressor region
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DOI：
10.1006/geno.1999.6101
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发表时间：
2000-02-15
期刊：
影响因子：
4.4
通讯作者：
Jenkins, RB
中科院分区：
文献类型：
--
作者：
Smith, JS;Tachibana, I;Jenkins, RB
染色体19q臂等位基因缺失在人类弥漫性胶质瘤中是一种常见的事件，提示它含有肿瘤抑制基因。最近的缺失作图研究广泛地暗示了D19S241E和D19S596之间有1.6Mb的间隔，以及有限的肿瘤亚集，这表明该区域可能窄至150kb。着眼于这一较小的间隔，我们利用cDNA选择、外显子扩增和基因组测序的方法鉴定了三个新的转录本(EHD2、GLTSCR1和GLTSCR2)，并定位了两个已知基因(SEPW1和CRX)。在D19S241E-D19S596之间构建了一个包含19个转录本和两个EST标记的部分转录图谱。其中10个转录本，包括5个映射到150kb缺失区间的转录本，已经在一组等位基因缺失为19q的胶质瘤中进行了检测。到目前为止，在所检查的记录中还没有发现肿瘤特异性的改变。总的来说，这些数据应该有助于随后的努力，以识别和描述1.6-Mb间隔内的剩余抄本，(C)2000学术出版社。
Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval, (C) 2000 Academic Press.