BESTING AND ACTION POTENTIALS OF CULTURED CHICK EMBRYO SPINAL GANGLION CELLS
BESTING AND ACTION POTENTIALS OF CULTURED CHICK EMBRYO SPINAL GANGLION CELLS
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DOI：
10.1002/cne.901040207
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发表时间：
1956-01-01
影响因子：
2.5
通讯作者：
CRAIN, SM
中科院分区：
文献类型：
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作者：
CRAIN, SM
用Maximow玻片法培养7周的鸡胚脊神经节细胞，观察到电刺激引起的动作电位。在直接视觉控制下，通过微盐桥导线进行细胞内和细胞外记录。许多神经节细胞的膜动作电位的幅度达到80 - 95 mV，包括超过50 - 65 mV的静息电位范围30 - 40 mV的过冲。棘波出现在潜伏期之后，从几十毫秒到12毫秒不等，通常具有0.2 - 0.4毫秒的上升相和2 - 3毫秒的下降相。他们经常是由一个更缓慢上升的“prepotential”，其次是一个长期持续的阶段超极化。在低刺激强度小，分级阈下反应，有时诱发。后穗不应性，块与重复刺激，和阈下刺激的时间总和也被证明。在某些情况下，在微电极穿透后长达一小时的时间内，尖峰模式保持稳定，但通常在第一分钟内观察到明显的恶化。这些数据与神经元的基本模式特征基本一致。在观察到偏差的地方，证据表明，这些可能更多地是由于细胞形态和记录技术的特殊性，而不是体外显著的功能改变。
Action potentials in response to electrical stimulation were observed in chick embryo spinal ganglion cells which had been cultured by the Maximow slide method for as long as 7 weeks. Intracellular as well as extracellular recordings were made, via micro-salt-bridge leads, under direct visual control. The amplitudes of the membrane action potentials of many of the ganglion cells reached 80-95 mV, involving overshoots of 30-40 mV above a resting potential range of 50-65 mV. The spikes, which occurred after latencies varying from several tenths to 12 msec, often had rising phases of 0.2-0.4 msec and falling phases of 2-3 msec. They were frequently preceded by a more slowly rising "prepotential" and followed by a long-lasting phase of hyperpolarization. At low stimulus intensities small, graded subthreshold responses were sometimes evoked. Post-spike refractoriness, block with repetitive stimuli, and temporal summation of subliminal stimuli were also demonstrated. The spike pattern remained stable, in some cases, for periods up to one hour after microelectrode penetration, but marked deterioration was usually observed within the first minute. The data show good agreement with the basic pattern characteristic of neurons in general. Where deviations have been observed, the evidence indicates that these were probably due more to peculiarities in cell morphology and in recording technique than to significant functional alteration in vitro.