Regulative actions of dietary soy isoflavone on biological antioxidative system and lipid metabolism in rats
Regulative actions of dietary soy isoflavone on biological antioxidative system and lipid metabolism in rats
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DOI：
10.1021/jf0345898
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发表时间：
2004-03-24
影响因子：
6.1
通讯作者：
Osada, K
中科院分区：
文献类型：
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作者：
Kawakami, Y;Tsurugasaki, W;Osada, K
4周龄雄性SD大鼠饲喂含0.2%大豆异黄酮粉和不含0.2%大豆异黄酮粉的纯净日粮5周，以阐明其抗氧化作用和调节脂类代谢的直接作用。膳食大豆异黄酮能降低大鼠血清过氧化脂质水平。饲喂异黄酮组大鼠的肝脏和血清α-生育酚水平高于饲喂不含异黄酮组的大鼠。因此，在动物实验中，饲料中的大豆异黄酮表现出温和的抗氧化功能。饮食中的异黄酮代谢物可能是活性氧的清除剂。日粮大豆异黄酮能降低肝脏3-羟基-3-甲基戊二酰辅酶A还原酶活性，但对肝脏胆固醇水平没有影响。然而，食用大豆异黄酮会降低血清胆固醇和甘油三酯的水平。因此，膳食大豆异黄酮可能具有降胆固醇和降血脂的作用。此外，饲料中的大豆异黄酮降低了肝脏Delta6去饱和酶的活性。组织脂的Delta6减饱和指数((1 8：2(n=6)+18：3(n=6))120：4(n=6))显示，饲喂异黄酮组大鼠的组织脂Delta6降饱和度指数低于饲喂不含异黄酮组。这一作用可能有助于防止二十烷类化合物失衡所致的炎症反应。这些观察表明，大豆异黄酮的积极摄入可能通过其清除自由基的功能和降胆固醇作用来降低某些心血管疾病的风险。
Male Sprague-Dawley rats, 4 weeks of age, were fed purified diets either with or without 0.2% soy isoflavones rich powder for 5 weeks to elucidate their direct functions such as antioxidative action and regulation of lipid metabolism. Dietary soy isoflavones decreased serum lipid peroxide level in rats. Levels of liver and serum alpha-tocopherol were higher in the rats fed isoflavone than in those fed isoflavones-free diet. Thus, dietary soy isoflavones exhibited mild antioxidative function in this animal experiment. Isoflavone metabolites from diet may act as scavengers of reactive oxygen species. Dietary soy isoflavones lowered hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity, although liver cholesterol level was not modulated. However, the levels of serum cholesterol and triglyceride decreased by consumption of soy isoflavones. Therefore, dietary soy isoflavones may exhibit hypocholesterolemic and hypolipidemic functions. Moreover, dietary soy isoflavones lowered hepatic Delta6 desaturase activity. Reflecting this observation, Delta6 desaturation indices ((1 8:2(n = 6) + 18:3(n = 6))120:4(n = 6)) of tissue lipids tended to be lower in rats fed isoflavones than in those fed isoflavones free diet. This action may contribute to the prevention of inflammatory response by imbalance of eicosanoids. These observations suggest that the positive intake of soy isoflavones may reduce the risk of some cardiovasucular diseases through their radical scavenging function and hypocholesterolemic action.