Rb interacts with histone deacetylase to repress transcription
Rb interacts with histone deacetylase to repress transcription
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DOI：
10.1016/s0092-8674(00)80940-x
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发表时间：
1998-02-20
期刊：
影响因子：
64.5
通讯作者：
Dean, DC
中科院分区：
文献类型：
--
作者：
Luo, RX;Postigo, AA;Dean, DC
在此之前，我们发现Rb可以通过在启动子处结合和灭活转录因子来积极抑制细胞周期基因的转录。在这里，我们证明Rb还可以通过募集组蛋白去乙酰化酶来抑制含有E2F位点的内源性细胞周期基因的转录，该酶使启动子上的组蛋白去乙酰化，从而促进抑制转录的核小体的形成。Rb的这两种抑制机制是选择性的——一些启动子和转录因子被组蛋白去乙酰化酶的募集所阻断，而另一些启动子和转录因子对组蛋白去乙酰化酶的活性具有抗性，并直接被转录因子的抑制所抑制。
Previously, we found that Rb can actively repress transcription of cell cycle genes by binding and inactivating transcription factors at the promoter. Here, we demonstrate that Rb can also repress transcription of endogenous cell cycle genes containing E2F sites through recruitment of histone deacetylase, which deacetylates histones on the promoter, thereby promoting formation of nucleosomes that inhibit transcription. These two mechanisms of repression by Rb are selective-some promoters and transcription factors are blocked by this recruitment of histone deacetylase, whereas others are resistant to histone deacetylase activity and are repressed directly by inhibition of transcription factors.