Nutritional regulation of genes responsible for long-chain (C20-24) and very long-chain (>C24) polyunsaturated fatty acid biosynthesis in post-larvae of gilthead seabream (Sparus aurata) and Senegalese sole (Solea senegalensis)
Nutritional regulation of genes responsible for long-chain (C20-24) and very long-chain (>C24) polyunsaturated fatty acid biosynthesis in post-larvae of gilthead seabream (Sparus aurata) and Senegalese sole (Solea senegalensis)
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DOI：
10.1016/j.aquaculture.2020.735314
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发表时间：
2020-08
期刊：
影响因子：
4.5
通讯作者：
Miguel Torres;J. Navarro;I. Varó;Ó. Monroig;F. Hontoria
中科院分区：
文献类型：
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作者：
Miguel Torres;J. Navarro;I. Varó;Ó. Monroig;F. Hontoria
脂肪酰基延伸酶 Elovl4 是从长链多不饱和脂肪酸 (LC-PUFA) 生物合成极长链多不饱和脂肪酸 (VLC-PUFA) 的关键成分。因此，Elovl4以及参与LC-PUFA生物合成的其他延伸酶和去饱和酶基因（例如Elovl5、Fads2）的营养调节已被提议作为在集约化农业条件下增强LC-PUFA和VLC-PUFA内源性生产的策略。本研究旨在研究金鲷鱼和塞内加尔鳎鱼幼体中参与 VLC-PUFA（elovl4 亚型 a 和 b）和 LC-PUFA（fads2、elovl5）生物合成的基因的营养调节，通过使用鱼油和豆油的不同组合，饲喂三种含有分级浓度的日粮 LC-PUFA 的惰性微饲料。还检查了膳食 LC-PUFA 对存活、生长和脂肪酸组成的影响。结果表明，虽然没有观察到对存活率的影响，但在 30 天内饲喂最高 LC-PUFA 含量的饲料的鱼表现出更高的总长度和湿重。基因表达结果表明fads2、elovl5、elovl4a和delovl4b可受膳食LC-PUFA含量的调节。值得注意的是，我们的结果表明与每个物种相关的差异化营养调节。头部是这两种鱼类中研究程度最高的身体部位。
The fatty acyl elongases Elovl4 are pivotal components in the biosynthesis of very long-chain polyunsaturated fatty acids (VLC-PUFA) from long-chain polyunsaturated fatty acids (LC-PUFA). Thus, nutritional regulation of Elovl4, as well as other elongase and desaturase genes involved in LC-PUFA biosynthesis (e.g., Elovl5, Fads2) has been proposed as a strategy to enhance endogenous production of LC-PUFA and VLC-PUFA under intensive farming conditions. This study aimed at investigating the nutritional regulation of genes involved in the biosynthesis of VLC-PUFA (elovl4isoforms a and b) and LC-PUFA (fads2, elovl5) inSparus aurataandSolea senegalensispost-larvae fed three inert micro-diets with graded concentrations of dietary LC-PUFA by using different combinations of fish oil and soya oil. The effect of dietary LC-PUFA on survival, growth and fatty acid composition was also examined. The results denoted that, while no effects were observed in survival, fish fed the diet with the highest LC-PUFA content during 30 d showed a higher total length and wet weight. Gene expression results showed thatfads2, elovl5, elovl4aandelovl4bcan be regulated by dietary LC-PUFA content. Remarkably, our results denoted a differentialelovl4nutritional regulation associated to each species. The head is the body part studied with the highestelovl4transcripts in both fish species.