Molecular association of Arabidopsis RTH with its homolog RTE1 in regulating ethylene signaling
Molecular association of Arabidopsis RTH with its homolog RTE1 in regulating ethylene signaling
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DOI：
10.1093/jxb/erx175
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发表时间：
2017-05
影响因子：
6.9
通讯作者：
Fangfang Zheng;Xiankui Cui;Maximo Lisandro Rivarola;Ting Gao;Caren Chang;C. Dong
中科院分区：
文献类型：
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作者：
Fangfang Zheng;Xiankui Cui;Maximo Lisandro Rivarola;Ting Gao;Caren Chang;C. Dong
植物激素乙烯影响植物生长和发育过程中的许多生物过程。乙烯被内质网 (ER) 膜上的乙烯受体感知。 ETR1 乙烯受体受到跨膜蛋白 RTE1 的正向调节，RTE1 定位于内质网和高尔基体。 RTE1 基因家族在动物、植物和低等真核生物中是保守的。在拟南芥中，RTE1-HOMOLOG (RTH) 是拟南芥RTE1 基因家族的唯一同源物。拟南芥 RTH 在乙烯信号传导和植物生长中的调节功能很大程度上未知。本研究显示了拟南芥RTH基因表达模式、与ER和高尔基体的蛋白质共定位，以及拟南芥中RTH被敲除或过度表达时乙烯反应表型的改变。与rte1突变体相比，rth突变体对外源乙烯表现出较低的敏感性，而RTH过表达则赋予乙烯超敏感性。遗传分析表明拟南芥 RTH 可能不直接调节乙烯受体。 RTH 可以与 RTE1 发生物理相互作用，有证据表明 RTH 可能通过 RTE1 调节乙烯反应和信号传导。本研究增进了我们对拟南芥 RTE1 基因家族成员在乙烯信号传导中调节功能的理解。
The plant hormone ethylene affects many biological processes during plant growth and development. Ethylene is perceived by ethylene receptors at the endoplasmic reticulum (ER) membrane. The ETR1 ethylene receptor is positively regulated by the transmembrane protein RTE1, which localizes to the ER and Golgi apparatus. The RTE1 gene family is conserved in animals, plants, and lower eukaryotes. In Arabidopsis, RTE1-HOMOLOG (RTH) is the only homolog of the Arabidopsis RTE1 gene family. The regulatory function of the Arabidopsis RTH in ethylene signaling and plant growth is largely unknown. The present study shows Arabidopsis RTH gene expression patterns, protein co-localization with the ER and Golgi apparatus, and the altered ethylene response phenotype when RTH is knocked out or overexpressed in Arabidopsis. Compared with rte1 mutants, rth mutants exhibit less sensitivity to exogenous ethylene, while RTH overexpression confers ethylene hypersensitivity. Genetic analyses indicate that Arabidopsis RTH might not directly regulate the ethylene receptors. RTH can physically interact with RTE1, and evidence supports that RTH might act via RTE1 in regulating ethylene responses and signaling. The present study advances our understanding of the regulatory function of the Arabidopsis RTE1 gene family members in ethylene signaling.