RNA dynamics in live Escherichia coli cells
RNA dynamics in live Escherichia coli cells
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DOI：
10.1073/pnas.0404443101
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发表时间：
2004-08-03
影响因子：
11.1
通讯作者：
Cox, EC
中科院分区：
文献类型：
--
作者：
Golding, I;Cox, EC
我们描述了一种用于跟踪大肠杆菌中的RNA分子的方法，该方法对mRNA的单拷贝敏感，并且使用该方法，我们发现单个分子可以在活细胞中跟踪数小时。我们观察到不同的特征动态的RNA分子，所有的RNA在原核生物中的已知的生活史相一致：本地化的运动与布朗运动的RNA聚合物拴在其模板DNA，自由扩散，和聚合物链动力学的几个例子，似乎是一个组合的链波动和链伸长归因于RNA转录。我们还量化了一些动力学，如位移分布的宽度，扩散系数，链延伸率和分子数的分布，并将它们与已知的生物物理参数的E。coli系统。
We describe a method for tracking RNA molecules in Escherichia coli that is sensitive to single copies of mRNA, and, using the method, we find that individual molecules can be followed for many hours in living cells. We observe distinct characteristic dynamics of RNA molecules, all consistent with the known life history of RNA in prokaryotes: localized motion consistent with the Brownian motion of an RNA polymer tethered to its template DNA, free diffusion, and a few examples of polymer chain dynamics that appear to be a combination of chain fluctuation and chain elongation attributable to RNA transcription. We also quantify some of the dynamics, such as width of the displacement distribution, diffusion coefficient, chain elongation rate, and distribution of molecule numbers, and compare them with known biophysical parameters of the E. coli system.