cDNA cloning, expression, and mutagenesis of scytalone dehydratase needed for pathogenicity of the rice blast fungus, Pyricularia oryzae
cDNA cloning, expression, and mutagenesis of scytalone dehydratase needed for pathogenicity of the rice blast fungus, Pyricularia oryzae
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DOI：
10.1271/bbb.62.564
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发表时间：
1998-03-01
影响因子：
1.6
通讯作者：
Yamaguchi, I
中科院分区：
文献类型：
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作者：
Motoyama, T;Imanishi, K;Yamaguchi, I
我们从稻瘟病菌中分离纯化了鲨烯酮水解酶。梨孢霉Pyricularia nov.的cDNA序列，并根据其氨基酸序列克隆了其cDNA。推导的氨基酸序列与来自西瓜炭疽菌（Colletotrichum lagenarium）的镰刀菌酮水解酶的氨基酸序列同源性为62%。当黑色素合成发生时，该基因的表达在稳定期被转录诱导。我们构建了该酶在大肠杆菌中的异源表达系统，并对该系统进行了缺失分析，发现该酶的C-末端区域对该酶的功能是必需的。
We purified scytalone dehydratase from the rice blast fungus. Pyricularia oryzae, and cloned its cDNA on the basis of its amino acid sequence. The deduced amino acid sequence was 62% identical to the scytalone dehydratase from Colletotrichum lagenarium. The expression of this gene was induced transcriptionally in the stationary phase when melanin synthesis occurs. We constructed a heterologous expression system for the enzyme in Escherichia coli, did deletion analysis with this system, and found that a C-terminal region is essential for the enzyme function.