Conservation and genetic diversity of microsatellite loci in the genus Eucalyptus
Conservation and genetic diversity of microsatellite loci in the genus Eucalyptus
复制标题
DOI：
10.1071/bt9960331
复制
发表时间：
1996-01-01
影响因子：
1.1
通讯作者：
Moran, GF
中科院分区：
文献类型：
--
作者：
Byrne, M;MarquezGarcia, MI;Moran, GF
在桉树和其他六种桉树中发现了四个微卫星基因座。用(CA)(N)和(GA)(N)寡核苷酸探针筛选E.nitens的Sau3AI基因组DNA文库，并对阳性克隆进行测序，以鉴定二核苷酸重复序列。对5个种群20个无亲缘关系个体的遗传分析表明，所有基因座均为高度多态，平均每个座位有9.5个等位基因，平均杂合度为0.575。对3个亚属6个种中每个种的4个个体的分析表明，同一亚属内的种间微卫星座位完全保存，而两个主要亚属间50%的微卫星座位在两个主要亚属间保存。所有的引物都没有扩增出Corymbia亚属斑桉的微卫星座位。所有检测到的微卫星座位都是多态的。在物种间保守的高度多态的微卫星基因座将有助于绘制数量性状图、对育种品系进行指纹识别，以及需要对遗传变异进行精细分析的种群内研究。
Four microsatellite loci have been characterised in Eucalyptus nitens Maiden and in six other eucalypt species. The dinucleotide repeats were identified by screening a Sau3AI genomic DNA library from E. nitens with (CA)(n) and (GA)(n) oligonucleotide probes and sequencing the positive clones. Genetic analysis of 20 unrelated individuals from five populations of E. nitens showed all loci to be highly polymorphic with an average of 9.5 alleles per locus and an average heterozygosity of 0.575. Analysis of four individuals from each of six species from three subgenera showed complete conservation of microsatellite loci between species within the same subgenus, Symphyomyrtus, and conservation of 50% of loci across species between the two main subgenera, Symphyomyrtus and Monocalyptus. None of the primers amplified microsatellite loci in Eucalyptus maculata from the subgenus Corymbia. All microsatellite loci that were detected were polymorphic. Highly polymorphic microsatellite loci that are conserved across species will be useful for mapping quantitative traits, fingerprinting breeding lines, and for within-population studies requiring fine-scale analysis of genetic variation.