INTRACELLULAR DISTRIBUTION AND ROLE OF CARBONIC ANHYDRASE IN AVIAN (GALLUS DOMESTICUS) SHELL GLAND MUCOSA
INTRACELLULAR DISTRIBUTION AND ROLE OF CARBONIC ANHYDRASE IN AVIAN (GALLUS DOMESTICUS) SHELL GLAND MUCOSA
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DOI：
10.1016/0005-2744(68)90085-5
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
SCHRAER, H
中科院分区：
文献类型：
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作者：
BERNSTEIN, RS;NEVALAINEN, T;SCHRAER, H
碳酸酐酶（碳酸氢裂解酶，EC 4.2.1.1）已在禽类（鸡内鸡）壳腺的粘膜细胞中进行了研究，并发现它是这些细胞的可溶性成分。线粒体和微粒体部分显示出非常低且无波动的碳酸酐酶活性水平。这些发现支持该酶具有非膜附着的细胞内作用。肌内注射特定抑制剂乙酰唑胺与粘膜细胞酶活性的降低和蛋壳重量的降低相关。这些结果被解释为表明壳腺粘膜细胞中的酶与 CaCO3 密切相关。沉积。正常和乙酰唑胺处理的蛋鸡的壳腺粘膜的精细结构没有显示出与碳酸酐酶抑制相关的超结构变化。讨论了碳酸酐酶在壳腺粘膜中的作用模式模型。
The enzyme, carbonic anhydrase (carbonate hydro-lyase, EC 4.2.1.1), has been studied in the mucosal cells of the avian (Gallus domesticus) shell gland and has been found to be a soluble constituent of these cells. Mitochondrial and micro -somal fractions showed very low and non-fluctuating levels of carbonic anhydrase activity. These findings support a non-membrane-attached, intracellular role for the enzyme. Intramuscular injections of the specific inhibitor, acetazolamide, were correlated with decreases of enzymatic activity in the mucosal cells and with decreases in egg shell weight. These results have been interpreted to indicate an intimate association of the enzyme in the shell gland mucosal cells with CaCO3. deposition. The fine structure of the shell gland mucosa in normal and acetazolamide-treated laying hens did not reveal ultra-structural changes in conjunction with carbonic anhydrase inhibition. A model of the mode of action of carbonic anhydrase in the shell gland mucosa is discussed.