Multifunctional factor ENY2 is associated with the THO complex and promotes its recruitment onto nascent mRNA
Multifunctional factor ENY2 is associated with the THO complex and promotes its recruitment onto nascent mRNA
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DOI：
10.1101/gad.550010
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发表时间：
2010-01-01
影响因子：
10.5
通讯作者：
Georgieva, Sofia G.
中科院分区：
文献类型：
--
作者：
Kopytova, Daria V.;Orlova, Anastasija V.;Georgieva, Sofia G.
Metazoan E(y)2/ENY2是一种多功能蛋白，对转录激活和mRNA输出很重要，是SAGA/TFTC和mRNA输出复合体AMEX的一个组成部分。在这里，我们发现果蝇中的ENY2也稳定地与THO相关，THO是参与mRNP生物发生的复合物。ENY2-THO复合体是正常果蝇发育所必需的，独立于SAGA和AMEX起作用。ENY2和THO在hsp70基因激活后到达其转录区，ENY2在THO募集中起重要作用。ENY2和THO与延长RNA聚合酶II无直接关联。ENY2和THO的招募发生在新生mRNA的合成后立即被装载到新生mRNA上，而AMEX成分Xmas-2则在后期被装载到mRNA上。ENY2或THO的敲低，但SAGA或AMEX的敲低不会影响转录本3'端的处理。因此，ENY2作为控制基因表达顺序步骤的几个蛋白复合物的共享亚基，在这些步骤的协调中起着重要作用。
Metazoan E(y)2/ENY2 is a multifunctional protein important for transcription activation and mRNA export, being a component of SAGA/TFTC and the mRNA export complex AMEX. Here, we show that ENY2 in Drosophila is also stably associated with THO, the complex involved in mRNP biogenesis. The ENY2-THO complex is required for normal Drosophila development, functioning independently on SAGA and AMEX. ENY2 and THO arrive on the transcribed region of the hsp70 gene after its activation, and ENY2 plays an important role in THO recruitment. ENY2 and THO show no direct association with elongating RNA polymerase II. Recruitment of ENY2 and THO occurs by their loading onto nascent mRNA, apparently immediately after its synthesis, while the AMEX component Xmas-2 is loaded onto mRNA at a later stage. Knockdown of either ENY2 or THO, but not SAGA or AMEX, affects the processing of the transcript's 3' end. Thus, ENY2, as a shared subunit of several protein complexes governing the sequential steps of gene expression, plays an important role in the coordination of these steps.