Induction of immunodeficiency virus-specific immune responses in rhesus monkeys following gene gun-mediated DNA vaccination
Induction of immunodeficiency virus-specific immune responses in rhesus monkeys following gene gun-mediated DNA vaccination
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DOI：
10.1111/j.1600-0684.1996.tb00021.x
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发表时间：
1996-06-01
影响因子：
0.7
通讯作者：
Haynes, JR
中科院分区：
文献类型：
--
作者：
Fuller, DH;MurpheyCorb, M;Haynes, JR
利用Accell(R)基因传递系统（基因枪）将包裹HIV-1（LAI）和SIVmac239表达构建物的金颗粒传递到恒河猴表皮，引发环境和gag特异性体液反应。使用cmv内含子A启动子驱动的SIVmac239 gp160和gp120载体，每剂量1微克的载体DNA足以诱导猴子产生免疫反应。在设计最佳的DNA免疫方案时，包括载体的身份、免疫间隔时间、免疫次数和每次免疫的DNA量在内的几个参数都很重要。此外，使用低效率表达载体的基因枪DNA免疫是一种有效的手段，在重组亚基增强后，在猕猴体内诱导强烈的抗体反应。
The Accell(R) gene delivery system (gene gun) was used to deliver gold particles coated with HIV-1(LAI) and SIVmac239 expression constructs into the epidermis of rhesus macaques, resulting in the elicitation of env- and gag-specific humoral responses. One microgram of vector DNA per dose was sufficient to induce immune responses in monkeys using SIVmac239 gp160 and gp120 vectors driven by the CMV-intron A promoter. Several parameters, including the identity of the vector, the length of the rest period between immunizations, the number of immunizations, and the amount of DNA per immunization, are all important in designing an optimal DNA immunization regimen. In addition, gene gun-based DNA immunization using low efficiency expression vectors is an effective means of priming for the induction of vigorous antibody responses in macaques following boosting with recombinant subunits.