A new method for measuring red blood cell aggregation using pattern recognition techniques on backscattered ultrasound Doppler signals.
A new method for measuring red blood cell aggregation using pattern recognition techniques on backscattered ultrasound Doppler signals.
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一种利用反向散射超声多普勒信号的模式识别技术测量红细胞聚集的新方法。
DOI：
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发表时间：
1999
影响因子：
2.1
通讯作者：
D. Koutsouris
中科院分区：
文献类型：
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作者：
E. Karabetsos;C. Papaodysseus;D. Koutsouris
为了研究体外红细胞聚集的重要现象，本实验室独创了体外超声多普勒技术。在用这种新技术进行的实验中，通过添加不同分子量和浓度的右旋糖酐来检测红细胞聚集，以获得大范围的红细胞压积值。因此，建立了一个大型的后向散射超声多普勒信号数据库。在这些信号中，应用了各种模式识别方法，主要是形态学和统计方法。分析结果表明，获得的后向散射信号的包络度、能量和面积特征是红细胞聚集程度的良好指标。通过这种方法，我们已经能够：(a)建立各种右旋糖酐的存在与聚合程度之间的定量关系。(b)确定在不同的实验溶液中形成的聚集体的大小分布。(c)获得超声后向散射与相应观察到的聚集体的实际尺寸之间最准确的关系。
In order to study the important phenomenon of aggregation of red blood cell in vitro, an original ultrasound Doppler in vitro technique has been designed and developed in our laboratory. In the experiments held with this new technique, erythrocyte aggregation was examined by adding dextrans of different molecular weights and concentrations for a wide range of hematocrit values. Consequently, a large data base of backscattered ultrasound Doppler signals is created. In these signals, various pattern recognition methods have been applied, mainly morphological and statistical ones. It results from the employed analysis that the envelope, energy and area characteristics of the obtained backscattered signals are quite good indicators of the degree of RBCs aggregation. In this way, we have been able to: (a) Establish a quantitative relation between the presence of various dextrans and the degree of aggregation. (b) Determine the distribution of the size of the formed aggregates in the various experimental solutions. (c) Obtain a most accurate relation between ultrasound backscattering and actual size of the corresponding observed aggregates.