SPECIFIC DNA-RNA HYBRID BINDING BY ZINC-FINGER PROTEINS
SPECIFIC DNA-RNA HYBRID BINDING BY ZINC-FINGER PROTEINS
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DOI：
10.1126/science.7536342
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发表时间：
1995-04-14
期刊：
影响因子：
56.9
通讯作者：
BERG, JM
中科院分区：
文献类型：
--
作者：
SHI, YG;BERG, JM
Cys(2)His(2) 类型的锌指蛋白代表一大类蛋白质，人们认为这些蛋白质通过与 DNA 的特异性相互作用发挥作用。由锌指蛋白-DNA复合物的结构特征驱动的实验表明，某些锌指蛋白结合DNA-RNA杂交体的亲和力相当于或大于DNA双链体的亲和力。锌指蛋白和 DNA-RNA 杂交体之间的相互作用取决于哪条链是 RNA，并且是序列特异性的。因此，应考虑锌指蛋白的生物学作用与 DNA-RNA 杂交体的相互作用。
Zinc finger proteins of the Cys(2)His(2) type represent a large class of proteins that have been assumed to function by means of specific interactions with DNA. Experiments motivated by structural characteristics of zinc finger protein-DNA complexes revealed that certain zinc finger proteins bound DNA-RNA hybrids with affinities comparable to or greater than those for DNA duplexes. The interactions between the zinc finger proteins and the DNA-RNA hybrids were dependent on which strand was RNA and were sequence-specific. Thus, interactions with DNA-RNA hybrids should be considered with regard to the biological roles of zinc finger proteins.