Chromosome 1p and 14q FISH analysis in clinicopathologic subsets of meningioma: Diagnostic and prognostic implications
Chromosome 1p and 14q FISH analysis in clinicopathologic subsets of meningioma: Diagnostic and prognostic implications
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DOI：
10.1093/jnen/60.6.628
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发表时间：
2001-06-01
影响因子：
3.2
通讯作者：
Perry, A
中科院分区：
文献类型：
--
作者：
Cai, DX;Banerjee, R;Perry, A
第二个最常见的报告基因异常脑膜瘤后22 q的损失是IP和14 q的缺失。为了评估这些染色体改变的潜在诊断和预后效用，我们研究了180例特征良好的脑膜瘤，使用双色荧光原位杂交（FISH）与DNA探针定位于1 p32。1p36，14q13。14q32我们的队列包括77例良性（I级）。74例非典型（II级）和29例间变性（III级）脑膜瘤。良性和非典型脑膜瘤进一步分为复发（尽管大体全切除）与非复发（至少随访10年）和有丝分裂活跃与脑浸润亚组。IP和14 q丢失分别在23%和31%的良性脑膜瘤、56%和57%的非典型脑膜瘤以及75%和67%的间变性脑膜瘤中被确定（1 p的p < 0.001：14 q的p = 0.004）。1 p/14 q联合缺失见于7%良性、39%非典型和63%间变性脑膜瘤（p < 0.001）。良性非复发性脑膜瘤比复发性脑膜瘤更不可能有149个缺失（17% vs 50%，p = 0.013）。有一种趋势，即14 q缺失的间变性脑膜瘤和1 p/14 q缺失的非典型脑膜瘤的总体生存率较差，但均未达到统计学意义。我们的结论是，1 p和14 q缺失与组织学分级的增加高度相关，并在脑膜瘤的肿瘤进展中发挥重要作用。此外。14 q FISH分析可能有助于评估组织学良性脑膜瘤的复发风险。
The second most frequently reported genetic abnormalities in meningiomas after 22q loss are deletions of Ip and 14q. To assess the potential diagnostic and prognostic utility of these chromosomal alterations, we studied 180 well-characterized meningiomas using dual-color fluorescence in situ hybridization (FISH) with DNA probes localized to 1p32. 1p36, 14q13. and 14q32. Our cohort consisted of 77 benign (grade I). 74 atypical (grade II), and 29 anaplastic (grade III) meningiomas. Benign and atypical meningiomas were further stratified into subsets of recurring (despite gross total resection) vs non-recurring (at least 10 yr of follow-up) and mitotically active vs brain invasive subsets, respectively. Losses of Ip and 14q losses were identified in 23% and 31% of benign, 56% and 57% of atypical, and 75% and 67% of anaplastic meningiomas, respectively (p < 0.001 for 1p: p = 0.004 for 14q). Combined 1p/14q deletions were encountered in 7% benign, 39% atypical, and 63% anaplastic meningiomas (p < 0.001). Benign non-recurring meningiomas were less likely to harbor 149 deletions than recurring examples (17% vs 50%, p = 0.013). There was a trend for anaplastic meningiomas with 14q deletions and atypical meningiomas with combined 1p/14q deletions to have poorer overall survivals, though neither reached statistical significance. We conclude that 1p and 14q deletions are highly associated with increasing histologic grade and play an important role in meningioma tumor progression. Furthermore. 14q FISH analysis may aid in assessing recurrence risk in histologically benign meningiomas.