Electrospinning of collagen and elastin for tissue engineering applications
Electrospinning of collagen and elastin for tissue engineering applications
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DOI：
10.1016/j.biomaterials.2005.06.024
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发表时间：
2006-02-01
期刊：
影响因子：
14
通讯作者：
Feijen, J
中科院分区：
文献类型：
--
作者：
Buttafoco, L;Kolkman, NG;Feijen, J
胶原蛋白和/或弹性蛋白的网成功地制备通过静电纺丝从水溶液。流速、外加电场、收集距离和起始溶液的组成决定了所得纤维的形态。添加PEO（M-w = 8 × 10（6））和NaCl对于纺制连续和均匀的纤维总是必要的。将胶原蛋白和弹性蛋白的混合物纺成纤维，其中单个组分不能通过SEM区分。增加弹性蛋白含量确定了纤维直径从220 nm增加到600 nm。连续生产纤维所需的电压取决于起始溶液的组成，但总是在10和25 kV之间。在这些条件下，可以形成非织造网，并且通过使用旋转管状心轴作为收集器来获得构成网的纤维的部分取向。胶原蛋白/弹性蛋白（1：1）通过用N-（3-二甲基氨基丙基）-N '-乙基碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）交联来稳定网片。这种处理提供了具有高热稳定性（Td = 79 ℃）的材料，而不改变其原始形态。交联后，PEO和NaCl完全浸出。培养14天后，平滑肌细胞在交联补片顶部生长为汇合层。(c)2005爱思唯尔有限公司保留所有权利。
Meshes of collagen and/or elastin were successfully prepared by means of electrospinning from aqueous solutions. Flow rate, applied electric field, collecting distance and composition of the starting solutions determined the morphology of the obtained fibres. Addition of PEO (M-w = 8 x 10(6)) and NaCl was always necessary to spin continuous and homogeneous fibres. Spinning a mixture of collagen and elastin resulted in fibres in which the single components could not be distinguished by SEM. Increasing the elastin content determined an increase in fibres diameters from 220 to 600 nm. The voltage necessary for a continuous production of fibres was dependent on the composition of the starting solution, but always between 10 and 25 kV. Under these conditions, non-woven meshes could be formed and a partial orientation of the fibres constituting the mesh was obtained by using a rotating tubular mandrel as collector. Collagen/elastin (1: 1) meshes were stabilized by crosslinking with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). This treatment afforded materials with a high thermal stability (T-d = 79 degrees C) without altering their original morphology. Upon crosslinking PEO and NaCl were fully leached out. Smooth muscle cells grew as a confluent layer on top of the crosslinked meshes after 14d of culture. (c) 2005 Elsevier Ltd. All rights reserved.