Transport of Protein by Goldfish Optic Nerve Fibers
Transport of Protein by Goldfish Optic Nerve Fibers
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金鱼视神经纤维运输蛋白质
DOI：
10.1126/science.157.3785.196
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发表时间：
1967
期刊：
影响因子：
56.9
通讯作者：
B. Grafstein
中科院分区：
文献类型：
--
作者：
B. Grafstein
将氚化亮氨酸注射到金鱼的眼睛后，神经节细胞体合成的放射性蛋白质以平均每天0.4毫米的速度沿视轴突向下移动。这些轴突末端所在的视顶盖的放射自显影显示，早在注射后 24 小时，在包含视轴突的顶盖层中的放射性水平上升到背景水平以上之前，包含轴突末端的层就已经被严重标记。注射后约 48 小时，末端的放射性达到最大值，并在此后至少 23 天保持大致恒定，而在同一时间间隔内，随着缓慢移动的蛋白质成分进入纤维层，纤维层的放射性显着增加。因此，似乎存在一种特殊的机制，可以将蛋白质从细胞体快速转运到突触末端，以及蛋白质沿着轴突的较慢运动。
After tritiated leucine was injected into the eye of goldfish, radio-active protein synthesized by the ganglion cell bodies moved down the optic axons at an average rate of 0.4 mm per day. Radioautograms of the optic tectum in which these axons end show that, as early as 24 hours after the injection, before the radioactivity in the tectal layer containing the optic axons had risen above background level, the layer containing the axon terminals was already heavily labeled. The radioactivity in the terminals reached a maximum about 48 hours after the injection and remained approximately constant for at least 23 days thereafter, whereas the radioactivity in the fiber layer increased significantly during the same interval, as the slowly moving protein component entered it. Thus there appears to be a special mechanism for rapid transport of protein from the cell body to the synaptic terminals, as well as a slower movement of protein down the axon.