Number and density of AMPA receptors in single synapses in immature cerebellum
Number and density of AMPA receptors in single synapses in immature cerebellum
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DOI：
10.1523/jneurosci.4256-04.2005
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发表时间：
2005-01-26
影响因子：
5.3
通讯作者：
Shigemoto, R
中科院分区：
文献类型：
--
作者：
Tanaka, J;Matsuzaki, M;Shigemoto, R
突触中离子型受体的数量是决定快速传递功效的重要因素。我们估计功能AMPA受体在单个突触后位点的谷氨酸双光子uncaging和非稳态波动分析在未成熟的大鼠浦肯野细胞（PC），它收到一个单一类型的兴奋性输入攀爬纤维的组合。在记录突触的突触后膜特化的领域进行了测量，通过重建的连续切片。功能性AMPA受体的数量与突触面积成正比，其密度类似于1280个受体/mum（2）。此外，高度敏感的冷冻断裂复制标记显示，在未成熟的PC中，突触部位的AMPA受体免疫金颗粒密度均匀（910 +/- 36颗粒/μ m（2）），突触外部位的密度低得多（19 +/- 2颗粒/μ m（2））。我们的研究结果表明，在这个发育中的突触，传输的功效是由突触面积。
The number of ionotropic receptors in synapses is an essential factor for determining the efficacy of fast transmission. We estimated the number of functional AMPA receptors at single postsynaptic sites by a combination of two-photon uncaging of glutamate and the nonstationary fluctuation analysis in immature rat Purkinje cells (PCs), which receive a single type of excitatory input from climbing fibers. Areas of postsynaptic membrane specialization at the recorded synapses were measured by reconstruction of serial ultrathin sections. The number of functional AMPA receptors was proportional to the synaptic area with a density of similar to1280 receptors/mum(2). Moreover, highly sensitive freeze-fracture replica labeling revealed a homogeneous density of immunogold particles for AMPA receptors in synaptic sites ( 910 +/- 36 particles/mum(2)) and much lower density in extrasynaptic sites ( 19 +/- 2 particles/mum(2)) in the immature PCs. Our results indicate that in this developing synapse, the efficacy of transmission is determined by the synaptic area.