Separation of andrographolide and neoandrographolide from the leaves of Andrographis paniculata using high-speed counter-current chromatography
Separation of andrographolide and neoandrographolide from the leaves of Andrographis paniculata using high-speed counter-current chromatography
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DOI：
10.1016/s0021-9673(02)01831-9
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发表时间：
2003-01-10
影响因子：
4.1
通讯作者：
Winterhalter, P
中科院分区：
文献类型：
--
作者：
Du, QZ;Jerz, G;Winterhalter, P
采用逆流色谱法成功分离了穿心莲叶中具有生物活性的穿心莲内酯和新穿心莲内酯。采用水-甲醇-乙酸乙酯-正己烷（2.5：2.5：4：1）溶剂系统，一次分离280分钟，得到99.9%穿心莲内酯189 mg和98.5%新穿心莲内酯9.5 mg。通过电喷雾MS、一维NMR实验、圆二色谱、旋光度色散和比旋光度[α]（D）进行结构确认。(C)2002 Elsevier Science B. V.保留所有权利。
The bioactive diterpenes andrographolide and neoandrographolide from the leaves of Andrographis paniculata NEES (Acanthaceae) were successfully separated by counter-current chromatography. A single 280-min separation yielded 189 mg of 99.9% andrographolide and 9.5 mg of 98.5% neoandrographolide applying water-methanol-ethyl acetate-n-hexane (2.5:2.5:4:1) solvent system. Structure confirmation was done by electrospray MS, one-dimensional NMR experiments, circular dichroism, optical rotation dispersion, and specific optical rotation [alpha](D). (C) 2002 Elsevier Science B.V. All rights reserved.