Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components
Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components
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DOI：
10.1016/j.dci.2007.09.003
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Sharif, Shayan
中科院分区：
文献类型：
--
作者：
Brisbin, Jennifer T.;Zhou, Huaijun;Sharif, Shayan
嗜酸乳杆菌已被证明在包括鸡在内的许多物种中发挥免疫刺激活性。为了研究这一现象的分子机制，我们研究了免疫系统基因在鸡盲肠扁桃体和脾单核细胞中的时空表达，以响应L.嗜酸乳杆菌使用低密度鸡免疫系统微阵列，我们发现盲肠扁桃体细胞比脾细胞对细菌刺激的反应更快，盲肠扁桃体细胞的最有效刺激是DNA，脾细胞的最有效刺激是细菌细胞瓦特成分。我们还发现，在脾细胞和盲肠扁桃体细胞中，STAT2和STAT4基因被高度诱导。鉴于盲肠扁桃体细胞和盲肠细菌之间的密切相互作用，我们进一步研究了STAT2和STAT4信号通路在细菌DNA的细胞反应中的参与。我们的研究结果显示，在盲肠扁桃体细胞中，经L.嗜酸乳杆菌DNA (c)2007爱思唯尔有限公司保留所有权利。
Lactobacillus acidophilus has been shown to exert immunostimulating activities in a number of species, including the chicken. To examine the molecular mechanisms of this phenomenon, we investigated spatial and temporal expression of immune system genes in chicken cecal tonsil and spleen mononuclear cells in response to structural constituents of L. acidophilus. Using a low-density chicken immune system microarray, we found that cecal tonsil cells responded more rapidly than spleen cells to the bacterial stimuli, with the most potent stimulus for cecal, tonsil cells being DNA and for splenocytes being the bacterial cell watt components. We also discovered that in both splenocytes and cecal tonsil cells, STAT2 and STAT4 genes were highly induced. Given the close interactions between cecal tonsil cells and commensal bacteria, we further examined the involvement of STAT2 and STAT4 signaling pathways in cellular responses to bacterial DNA. Our results revealed that the expression of STAT2, STAT4, IL-18, MyD88, IFN-alpha, and IFN-gamma genes were up-regulated in cecal tonsil cells after treatment with L. acidophilus DNA. (c) 2007 Elsevier Ltd. All rights reserved.