Phylogenetic analysis of Festuca-Lolium complex using SRAP markers
Phylogenetic analysis of Festuca-Lolium complex using SRAP markers
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DOI：
10.1007/s10722-015-0324-5
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Zhang, Xin Quan
中科院分区：
文献类型：
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作者：
Cheng, Yajuan;Ma, Xiao;Zhang, Xin Quan
黑麦草属和高羊茅属已被视为饲料生产和草坪工业中最重要的温带禾本科植物。这两个属无疑代表了一个密切相关的物种的复合体。利用序列相关扩增多态性（SRAP）分子标记对32种59份苜蓿-羊茅复合体材料的遗传多样性和亲缘关系进行了研究。22对引物共扩增出269条多态性条带，平均每个位点检测到12.23个等位基因。所研究的物种之间的平均多态性率（P）为100%，表明其高度的遗传多样性。POPGENE和AMOVA分析结果表明，属间多样性和属间变异均小于50%。用NTR 4和MEGA法以及PCO法构建的系统树图一致，清晰地反映了黑麦草属、羊茅亚属、Schedonorus和Leucopoa之间的亲缘关系。这一结果有力地支持了先前的“阔叶”和“细叶”分支的形态学划分，并再次证明了Lolium属和Schedonorus亚属之间的密切关系。本研究还建议将F.马伊雷湾并通过将银合欢组和短芒组移至不同的亚属来划分银合欢亚属。所描述的分析方法为确定这些远交种的亲缘关系提供了新的方法。
The Lolium and Festuca genera have been treated as the most important groupings of temperate grasses in forage production and the turf industry. The two genera undoubtedly represent a closely allied complex of related species. The objective of this study was to obtain an overview of the diversity and genetic relationships among 59 accessions of 32 species of the Lolium-Festuca complex using sequence related amplified polymorphism (SRAP) markers. In total, 22 primer combinations amplified 269 polymorphic bands which were detected with an average of 12.23 alleles per SRAP locus. The average polymorphic rate (P) between the species studied was 100 %, demonstrating their high degree of genetic diversity. According to the POPGENE and AMOVA analyses, the inter-genera diversity and the variance between the two genera were both under 50 %. The dendrograms derived either by NTSYS or MEGA in addition to the PCO were consistent and clearly illustrated the relationships among representatives of the Lolium genus as well as the subgenus Festuca, Schedonorus and Leucopoa within the Festuca genus. The result of this study strongly supported the previous morphological separation into a "broad leaved'' and "fine-leaved'' clades, and it once again demonstrated the close relationship between the Lolium genus and the Schedonorus subgenus. This study also suggested the inclusion of F. mairei in subg. Schedonorus and to divide subgenus Leucopoa by moving sect. Leucopoa and sect. Breviaristatae into different subgenera. The analysis method described provides new methodologies for determining the phylogeny of these outbreeding species.