[Sairei-to inhibits the production of endothelin-1 by nephritic glomeruli(2): alisols, possible candidates as active compounds].
[Sairei-to inhibits the production of endothelin-1 by nephritic glomeruli(2): alisols, possible candidates as active compounds].
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[Sairei-to 抑制肾小球产生内皮素-1(2)：泽泻醇，可能作为活性化合物的候选者]。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
H. Kawamura
中科院分区：
文献类型：
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作者：
T. Hattori;H. Nishimura;B. Makino;S. Shindo;H. Kawamura
我们先前曾报道，日本草药柴苓汤（TJ - 114）可抑制抗肾小球基底膜（GBM）肾炎大鼠内皮素 - 1的产生，而组成TJ - 114的12种草药之一的泽泻（日语为Takusha）可能是其作用的原因。为了进一步阐明TJ - 114的抗肾炎成分，我们利用抗GBM肾炎大鼠研究了Takusha提取物对各种参数的影响，包括体内外肾小球内皮素 - 1的产生。当将100%甲醇 - 甲醇和50%甲醇 - 甲醇组分（31.3微克/毫升或更高浓度）添加到源于肾炎大鼠的肾小球细胞培养物中时，它们强烈抑制培养基中内皮素 - 1浓度的升高。此外，口服100%甲醇 - 甲醇组分（30毫克/千克）可改善肾炎大鼠的蛋白尿、收缩压升高以及组织病理学参数的变化。口服100%甲醇 - 甲醇组分可抑制肾炎大鼠肾小球内皮素 - 1表达的增加以及源于肾炎大鼠的肾小球细胞培养物中内皮素 - 1产生的增加。100%甲醇 - 甲醇组分的主要成分泽泻醇A和B可抑制源于肾炎大鼠的肾小球细胞在体外产生内皮素 - 1。口服泽泻醇B（30毫克/千克）可阻止肾小球内皮素 - 1的表达以及培养的肾炎肾小球细胞内皮素 - 1产生的增加。口服泽泻醇B还可改善肾炎大鼠的蛋白尿、收缩压升高以及组织病理学参数的变化。这些结果表明，TJ - 114因抑制内皮素 - 1产生而具有的抗肾炎作用可能归因于Takusha中的泽泻醇。
We have previously reported that Sairei-to (TJ-114), a Japanese herbal medicine, prevented the production of endothelin-1 in anti-GBM nephritic rats, and that Alismatis Rhizoma (Takusha in Japanese), one of the twelve herbs composing TJ-114, might be responsible for the action. In order to further clarify the antinephritic components of TJ-114, we investigated the effects of Takusha extracts on various parameters, including endothelin-1 production of glomeruli in vitro and in vivo using anti-GBM nephritic rats. MeOH-100% MeOH and MeOH-50% MeOH fractions (31.3 microgram/ml or higher) strongly inhibited an increase in endothelin-1 concentration in culture medium when they were added to a culture of glomerular cells derived from nephritic rats. In addition, oral administration of the MeOH-100% MeOH fraction (30 mg/kg) ameliorated the proteinuria, increase in systolic blood pressure and changes in histopathological parameters in nephritic rats. Oral administration of the MeOH-100% MeOH fraction inhibited increase in endothelin-1 expression in the glomeruli of nephritic rats and in endothelin-1 production by a culture of glomerular cells derived from the nephritic rats. Alisols A and B, the main constituents of the MeOH-100% MeOH fraction, inhibited in vitro endothelin-1 production by glomerular cells derived from the nephritic rats. Oral administration of alisol B (30 mg/kg) prevented the endothelin-1 expression by glomeruli and the increase in endothelin-1 production by cultured nephritic glomerular cells. Oral administration of alisol B also ameliorated the proteinuria, the increase in systolic blood pressure and the changes in histopathological parameters in the nephritic rats. These results indicate that the antinephritic action of TJ-114, resulting from the inhibition of endothelin-1 production, may be attributed to the alisols in Takusha.