Effects of low power laser irradiation on intracellular calcium and histamine release in RBL-2H3 mast cells
Effects of low power laser irradiation on intracellular calcium and histamine release in RBL-2H3 mast cells
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DOI：
10.1111/j.1751-1097.2007.00116.x
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发表时间：
2007-07-01
影响因子：
3.3
通讯作者：
Zhou, Lu-Wei
中科院分区：
文献类型：
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作者：
Yang, Wen-Zhong;Chen, Ji-Yao;Zhou, Lu-Wei
尽管有报道称激光照射可促进皮肤伤口愈合，但其机制仍不清楚。由于发现肥大细胞在皮肤伤口处积聚，我们推测肥大细胞可能参与激光照射的生物效应。在这项工作中，肥大细胞 RBL-2H3 用于体外研究激光照射对细胞反应的影响。激光照射后，细胞内钙 ([Ca2+](i)) 量增加，随后组胺释放，分别通过 Fluo-3/AM 染色的共焦荧光显微镜和邻苯二甲醛染色的荧光光谱仪测量。组胺释放是由细胞外缓冲溶液通过阳离子通道蛋白瞬时受体电位香草酸 4 (TRPV4) 流入而增加的 [Ca2+](i) 介导的。 TRPV4抑制剂钌红(RR)可以有效阻止组胺的释放，表明TRPV4是激光照射响应的关键因素。这些肥大细胞诱导的反应可以解释激光照射对促进伤口愈合的生物效应，因为已知组胺具有加速伤口愈合的多种功能。
Although laser irradiation has been reported to promote skin wound healing, the mechanism is still unclear. As mast cells are found to accumulate at the site of skin wounds we hypothesized that mast cells might be involved in the biological effects of laser irradiation. In this work the mast cells, RBL-2H3, were used in ritro to investigate the effects of laser irradiation on cellular responses. After laser irradiation, the amount of intracellular calcium ([Ca2+](i)) was increased, followed by histamine release, as measured by confocal fluorescence microscopy with Fluo-3/AM staining and a fluorescence spectrometer with o-phthalaldehyde staining, respectively. The histamine release was mediated by the increment of [Ca2+](i) from the influx of the extracellular buffer solution through the cation channel protein, transient receptor potential vanilloid 4 (TRPV4). The TRPV4 inhibitor, Ruthenium Red (RR) can effectively block such histamine release, indicating that TRPV4 was the key factor responding to laser irradiation. These induced responses of mast cells may provide an explanation for the biological effects of laser irradiation on promoting wound healing, as histamine is known to have multi-functions on accelerating wound healing.