Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats.
Changes in miRNA in the lung and whole blood after whole thorax irradiation in rats.
复制标题
DOI：
10.1038/srep44132
复制
发表时间：
2017-03-17
影响因子：
4.6
通讯作者：
Medhora M
中科院分区：
文献类型：
--
作者：
Gao F;Liu P;Narayanan J;Yang M;Fish BL;Liu Y;Liang M;Jacobs ER;Medhora M
我们使用了大鼠模型的全胸部X射线照射，以分析肺和血液中的microRNA（miRNA）辐射后4周。通过下一代测序分析来自正常和辐射的Wistar大鼠肺和全血的miRNA，并在辐射后1、2、3和4周通过差异deRNA-seq鉴定辐射引起的变化。平均总读数/文库为2，703，137，平均88%映射到大鼠基因组。描述了大鼠血液和肺中100种最丰富的miRNA的详细概况。我们确定了放射后2周大鼠血液中4种miRNA，miR-144- 5 p，miR-144- 3 p，miR-142- 5 p和miR-19 a-3 p的上调，这些miRNA先前未显示在放射到肺后改变。免疫途径分析鉴定了炎症反应途径的信号传导。这些发现将支持早期检测方法的发展，以及放疗或放射性事故后患者的损伤和缓解机制。
We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described. We identified upregulation of 4 miRNA, miR-144-5p, miR-144-3p, miR-142-5p and miR-19a-3p in rat blood 2 weeks after radiation that have not previously been shown to be altered after radiation to the lung. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways. These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents.