Enhancement of anti-herpetic activity of glycyrrhizic acid by physiological proteins
Enhancement of anti-herpetic activity of glycyrrhizic acid by physiological proteins
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DOI：
10.1177/095632020101200206
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发表时间：
2001-03-01
影响因子：
--
通讯作者：
Pompei, R
中科院分区：
文献类型：
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作者：
Lampis, G;Deidda, D;Pompei, R
生物体液中存在的一些酶，如溶菌酶 (LYS) 和乳铁蛋白 (LAC)，已知具有抗菌和抗病毒活性，特别是针对疱疹病毒。本文将表明，它们与天然三萜，即甘草酸（GLA）的组合，在体外试验中可显着增强对 HSV1 的拮抗活性。通过计算三种化合物的每种组合的 FIC 指数（抑制浓度分数）并通过抑制组合效果的三维评估来进行数据加工，该效果表明了协同作用的百分比。 FIC指数等于或低于0.5表明两种物质之间具有显着的协同效应。考虑到每种单一化合物，病毒复制的50%抑制剂量(ID50)对于LAC为252+/-53μg/ml，对于LYS为497+/-165μg/ml，对于GLA为740+/-125μg/ml。 LAC和GLA的组合显示出明显的协同效应，FIC指数为0.08，对于某些剂量，增强活性差异高达1.5 log(10)（从约5.5 x 10(6)到10(5) pfu/ml）。 GLA 和 LYS 以及 LYS 和 LAC 的组合显示出不太显着的协同活性。这些发现得出这样的结论：一些生理蛋白质，即使在某些体液中通常存在的浓度下，也可能增强天然化合物（例如 GLA）的抗疱疹活性。
Some enzymes present in biological fluids, such as lysozyme (LYS) and lactoferrin (LAC), are known to possess antibacterial and antiviral activity, against herpesviruses in particular. It will be shown in this paper that their combination with a natural triterpene, namely glycyrrhizic acid (GLA), gives significant results in enhancing the antagonistic activity on HSV1 in in vitro assays. Data elaboration was carried out by calculation of the FIC index (fractional inhibitory concentration) for each combination of the three compounds and by a three-dimensional evaluation of the inhibiting combinatory effects, which indicated the percentage of the synergistic action. A FIC index equal to or below 0.5 demonstrated a significant synergistic effect between two substances. Considering each single compound, the 50% inhibiting doses on viral replication (ID50) were 252+/-53 mug/ml for LAC, 497+/-165 mug/ml for LYS and 740+/-125 mug/ml for GLA. The combination of LAC and GLA showed a clear synergistic effect, with a FIC index of 0.08 and a potentiating activity which, for some doses, was up to 1.5 log(10) of difference (from about 5.5 x 10(6) to 10(5) pfu/ml). The combinations of GLA and LYS, and LYS and LAC showed a less significant synergistic activity. These findings led to the conclusion that some physiological proteins, even at concentrations usually present in some body fluids, may enhance the anti-herpetic activity of a natural compound such as GLA.