THE UPTAKE OF GLUCOSE, FRUCTOSE AND SUCROSE INTO THE LOWER EPIDERMIS OF LEAF-DISKS OF PEA (PISUM-SATIVUM L CV ARGENTEUM)
THE UPTAKE OF GLUCOSE, FRUCTOSE AND SUCROSE INTO THE LOWER EPIDERMIS OF LEAF-DISKS OF PEA (PISUM-SATIVUM L CV ARGENTEUM)
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DOI：
10.1111/j.1469-8137.1993.tb03735.x
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发表时间：
1993-02-01
期刊：
影响因子：
9.4
通讯作者：
HALL, JL
中科院分区：
文献类型：
--
作者：
AKED, J;HALL, JL
豌豆突变体Pisum sativum L. CV.研究了叶表皮组织对糖的吸收特性。研究了黄瓜全叶圆片对1 mol m-3葡萄糖、果糖和蔗糖的吸收速率。Sativum cv.前进是非常相似的，在3小时内的吸收是线性的，葡萄糖比其他两种糖在两个品种。一个类似的模式吸收观察到下表皮的CV。Argenteum.此外，从1摩尔M-3 3-O-甲基葡萄糖的摄取明显慢于从1摩尔M-3葡萄糖。表皮组织的摄取率显示出一些每日变化;然而，摄取的基本模式是一致的。糖吸收的动力学分析显示存在两个运输组件。在浓度大于1摩尔m-3的线性成分占主导地位，果糖进入表皮速度比葡萄糖和蔗糖。外源性培养基的pH值从5增加到8引起葡萄糖、果糖和蔗糖的摄取速率轻微但稳定的下降;葡萄糖摄取的最佳pH值约为5。葡萄糖和果糖相互竞争，也与蔗糖竞争吸收到表皮中。然而，蔗糖不是葡萄糖和果糖的有效竞争者。0.05 mol m-3赤藓红B和1 mol m-3对氯汞苯磺酸（PCMBS）对葡萄糖、果糖和蔗糖的摄取抑制约20- 30%. 0.05 mol m-3的羰基氰间氯苯腙（CCCP）抑制葡萄糖和果糖的摄取（60- 70%），但基本上不抑制蔗糖的摄取。
The pea mutant, Pisum sativum L. cv. Argenteum, has been used to investigate the sugar uptake characteristics of leaf epidermal tissue. The uptake rates from either 1 mol m-3 glucose, fructose or sucrose into whole leaf discs of cv. Argenteum and of P. sativum cv. Onward were very similar; uptake was linear over a 3 h period, with glucose taken up faster than the other two sugars in both cultivars. A similar pattern for uptake was observed into the lower epidermis of cv. Argenteum. Furthermore, uptake from 1 mol m-3 3-O-methylglucose was markedly slower than from 1 Mol M-3 glucose. The epidermal tissue showed some day to day variation in uptake rates; however, the basic pattern of uptake was consistent. Kinetic analysis of sugar uptake revealed the presence of two transport components. At concentrations greater than 1 mol m-3 a linear component predominated with fructose entering the epidermis faster than glucose and sucrose. An increase in the pH of the exogenous medium from 5 to 8 caused a slight but steady drop in the rate of uptake of glucose, fructose and sucrose; glucose uptake had a pH optimum of about 5. Glucose and fructose competed with one another and also with sucrose for uptake into the epidermis. Sucrose was not, however, an effective competitor against glucose and fructose. The uptake of glucose, fructose and sucrose was inhibited by about 20-30 % by 0.05 mol m-3 erythrosin B and 1 mol m-3 parachloromercuribenzene sulphonic acid (PCMBS). Carbonyl cyanide m-chlorophenylhydrazone (CCCP) at 0.05 mol m-3 inhibited the uptake of glucose and fructose (60-70 %) but did not substantially inhibit sucrose uptake.