Transcriptional analysis of the IL-33 receptor suppression of tumourigenicity 2 and its effects on canine Type 2 T helper cells: a preliminary study
Transcriptional analysis of the IL-33 receptor suppression of tumourigenicity 2 and its effects on canine Type 2 T helper cells: a preliminary study
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IL-33 受体抑制致瘤性 2 的转录分析及其对犬 2 型 T 辅助细胞的影响：初步研究
DOI：
10.1111/vde.12512
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发表时间：
2017
影响因子：
1.4
通讯作者：
Maeda Sadatoshi
中科院分区：
文献类型：
--
作者：
Asahina Ryota;Nishida Hidetaka;Kamishina Hiroaki;Maeda Sadatoshi
白介素33与犬特应性皮炎的发病机制有关，特应性皮炎是一种2型辅助性T细胞(Th2)相关疾病。在人类中，IL-33通过抑制肿瘤致瘤性2(ST2)受体介导其生物学效应，ST2在Th2细胞上优先表达。IL-33对犬Th2细胞的作用尚不清楚。假设/目的ST2可能在犬Th2细胞上优先表达，IL-33可诱导这些细胞转录Th2细胞因子。用重组犬IL-33和/或重组人IL-2刺激Th2细胞，定量检测Th2细胞因子IL-4、IL-5、IL-13和IL-31的转录水平。Th2细胞在rcIL-33和rhIL-2刺激下也转录了IL-5和IL-13基因。结论和临床意义IL-33激活的犬Th2细胞通过产生IL-5和IL-13来增强Th2介导的炎症反应。
BackgroundInterleukin (IL)‐33 has been implicated in the pathogenesis of canine atopic dermatitis, a Type 2 T helper cell (Th2)‐associated disease. In humans, IL‐33 mediates its biological effects through the receptor suppression of tumourigenicity 2 (ST2), which is preferentially expressed on Th2 cells. The effects of IL‐33 on canine Th2 cells are unclear.Hypothesis/ObjectivesST2 may be preferentially expressed on canine Th2 cells; IL‐33 may induce the transcription of Th2 cytokines from these cells.AnimalsThree healthy dogs were used.MethodsThe transcription level ofst2was quantified in helper T cells, cytotoxic T cells and Th2 cells isolated from healthy dogs. The transcription levels of Th2 cytokines includingil‐4,il‐5,il‐13andil‐31were quantified in Th2 cells stimulated with recombinant canine (rc) IL‐33 and/or recombinant human (rh) IL‐2.ResultsTranscription ofst2was the strongest in Th2 cells. Th2 cells also transcribed the genes foril‐5andil‐13after being stimulated with rcIL‐33 and rhIL‐2.Conclusions and clinical importanceThese results indicate that canine Th2 cells activated by IL‐33 enhance Th2‐mediated inflammation through the production of IL‐5 and IL‐13.