The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.
The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.
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大肠杆菌中质子转位 NADH：泛醌氧化还原酶的基因座。
DOI：
10.1006/jmbi.1993.1488
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发表时间：
1993
影响因子：
5.6
通讯作者：
H. Weiss
中科院分区：
文献类型：
--
作者：
U. Weidner;Stephan Geier;A. Ptock;T. Friedrich;H. Leif;H. Weiss
利用聚合酶链式反应制备的DNA探针鉴定了大肠杆菌中质子转位NADH：泛醌氧化还原酶的基因座nuo。用于反应的引物源自线粒体 NADH 的 NAD(H) 结合亚基：泛醌氧化还原酶和真养产碱杆菌的 NAD(+) 还原氢化酶的共有序列。 nuo 基因座位于大肠杆菌染色体上的第 49.2 和 49.6 分钟之间，包含一组 14 个基因。它们的上游与类似于 sigma 70 依赖性启动子的序列接壤，下游与类似于 rho 独立终止子的序列接壤。所有来自 nuo 基因的 14 种蛋白质都与线粒体 NADH：泛醌氧化还原酶的亚基有关，其中发现了所有推测结合底物并含有氧化还原基团的亚基，以及所有七个线粒体编码的亚基。大肠杆菌酶似乎代表了质子转位 NADH 的最小形式：泛醌氧化还原酶。与其他细菌基因组和高等植物的叶绿体基因组相比，nuo位点的基因顺序是保守的。在某种程度上，基因顺序与编码亚基的拓扑排列相关。 nuo基因的排列进一步支持了NADH：泛醌氧化还原酶的模块化进化概念。
The gene locus nuo of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli was identified by means of a DNA probe made by the polymerase chain reaction. The primers used for the reaction were derived from consensus sequences of the NAD(H)-binding subunit of mitochondrial NADH: ubiquinone oxidoreductase and the NAD(+)-reducing hydrogenase of Alcaligenes eutrophus. The nuo locus lies between minutes 49.2 and 49.6 on the E. coli chromosome and contains a cluster of 14 genes. They are bordered upstream by a sequence resembling sigma 70-dependent promoters and downstream by a sequence resembling rho-independent terminators. All 14 proteins derived from the nuo-genes are related to subunits of mitochondrial NADH: ubiquinone oxidoreductase, among which all subunits presumed to bind the substrates and to harbour the redox groups are found, as well as all seven mitochondrially encoded subunits. The E. coli enzyme seems to represent the minimal form of a proton-translocating NADH: ubiquinone oxidoreductase. The gene order in the nuo locus is conserved in comparison with other bacterial genomes and the chloroplast genome of higher plants. To some extent, the gene order correlates with the topological arrangement of the encoded subunits. The conception of modular evolution of NADH: ubiquinone oxidoreductase is further supported by the arrangement of the nuo-genes.