Complete genome sequence analysis of tick-borne encephalitis viruses isolated in northeastern China
Complete genome sequence analysis of tick-borne encephalitis viruses isolated in northeastern China
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中国东北地区蜱传脑炎病毒全基因组序列分析
DOI：
10.1007/s00705-011-1031-y
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发表时间：
2011-08-01
影响因子：
2.7
通讯作者：
Zhu, Qing-Yu
中科院分区：
文献类型：
--
作者：
Si, Bing-Yin;Jiang, Tao;Zhu, Qing-Yu
蜱传脑炎病毒（TBEV）在人类中引起致命的脑炎，在许多欧洲和亚洲国家构成日益严重的公共卫生问题。TBEV目前在中国东北地区流行，但中国TBEV毒株的全基因组序列尚未报道。在2010年黑龙江省牡丹江市TBE暴发期间，从2例患者血清中分离到2株TBEV，测定其全序列，并与其他已知TBEV株进行比较。牡丹江分离物均由10,774个核苷酸组成，编码一个开放阅读框，编码3414个氨基酸的多聚蛋白，3'非翻译区（UTR）缺失364个核苷酸。根据E蛋白氨基酸序列和3 'UTR核苷酸序列进行系统进化分析，结果表明牡丹江分离株与Senzhang株和Sofjin-HO株亲缘关系较近，属于TBEV的远东亚型。这些发现为TBEV的实验室诊断和疫苗研制提供了依据。
Tick-borne encephalitis virus (TBEV) causes lethal encephalitis in humans, posing a growing public-health problem in many European and Asian countries. TBEV is currently endemic in northeastern China, but the complete genome sequences of Chinese TBEV strains have not been reported. During a TBE outbreak in 2010 in Mudanjiang City, Heilongjiang Province, China, two TBEV strains were isolated from serum samples of two patients, and the complete sequences were determined and compared with other known TBEV strains. Both Mudanjiang isolates consisted of 10,774 nucleotides and encoded a single open reading frame coding for a polyprotein of 3414 amino acids, and a unique deletion of 364 nucleotides in the 3’ untranslated region (UTR) was recorded. Phylogenetic analysis based on the amino acid sequence of the E protein and the nucleotide sequence of the 3’UTR revealed that the Mudanjiang isolates are closely related to the Senzhang and Sofjin-HO strains and belong to the Far Eastern subtype of TBEV. These findings provide insight into the evolutionary relationships among Chinese TBEV isolates and are useful for laboratory diagnosis and vaccine development for TBEV.