Investigations on the anther culturability of four German spring wheat cultivars and the influence of light on regeneration of green vs. albino plants.
Investigations on the anther culturability of four German spring wheat cultivars and the influence of light on regeneration of green vs. albino plants.
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DOI：
10.1111/j.1439-0523.1990.tb00450.x
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发表时间：
1990-09
期刊：
影响因子：
2
通讯作者：
G. Ziegler;K. Dressler;D. Hess
中科院分区：
文献类型：
--
作者：
G. Ziegler;K. Dressler;D. Hess
在四个德国春小麦品种（“Turbo”、“Kadett”、“Planet”和“Nandu”）的花药培养实验中，“Nandu”显示出最好的结果。在培养基 N6 上“Nandu”花药的响应率为 0.8%，在马铃薯 2 上响应花药的率为 3.7%。这与中国品种“Orofen”类似。使用培养基potato-2 可以获得比N6 更高的胚状体产量。用马铃薯2号培养基进行的实验表明，随着琼脂糖浓度的降低，花药反应、胚胎产生和再生增加。绿色幼苗的再生通过在黑暗中的分化得到改善，而在光照下的分化产生了超过 90% 的白化植物。
In anther culture experiments with four German spring wheat cultivars (‘Turbo’, ‘Kadett’, ‘Planet’ and ‘Nandu’), ‘Nandu’ showed the best results. The rate of responding anthers with ‘Nandu’ was 0.8 % on medium N6 and 3.7% on potato-2. This was similar to the Chinese cultivar ‘Orofen’. Higher yields of embryoids could be obtained with medium potato-2 than with N6. Experiments with medium potato-2 showed that anther response, production of embryos and regeneration increased with decreasing agarose concentration. Regeneration of green plantlets was improved by differentiation in the dark whereas differentiation under light yielded more than 90 % albino plants.