Sterilising embryos for transgenic chimaeras.
Sterilising embryos for transgenic chimaeras.
复制标题
转基因嵌合体胚胎的绝育。
DOI：
10.1080/00071669108417368
复制
发表时间：
1991
影响因子：
2
通讯作者：
K. Simkiss
中科院分区：
文献类型：
--
作者：
V. Aige;K. Simkiss
1. 进行了尝试用紫外线使家禽胚胎绝育的实验。这种绝育胚胎作为基因操纵生殖细胞的接受者是有用的。2. 胚胎的生发月牙在第4、8至10阶段暴露在校准的紫外光源下，孵育30秒、3分钟和10分钟。在长达6天的孵育期间，研究了致畸和不育效应。3. 仅仅通过打开外壳暴露胚胎就会产生许多异常和死亡。这些随胚胎年龄的增长而下降，但随辐照剂量的增加而增加。4. 虽然有充分的证据表明紫外线会导致细胞损伤，但胚胎的不孕率通常低于75%。
1. Experiments were undertaken to attempt to sterilise fowl embryos with ultraviolet light. Such sterilised embryos would be useful as recipients of genetically manipulated germ cells. 2. The germinal crescents of embryos were exposed to a calibrated UV source at stages 4 and 8 to 10 of incubation for 30 s, 3 min and 10 min. Teratological and sterility effects were studied at periods up to 6 d of incubation. 3. Simply exposing embryos by opening the shell produced a number of abnormalities and mortalities. These decreased with the age of the embryo but increased with the dosage of irradiation. 4. Although there was abundant evidence for UV-induced cell damage, the sterility of the embryos was usually less than 75%.