Circulating concentrations of sFlt1 (soluble fins-like tyrosine kinase 1) in fetal and maternal serum during pre-eclampsia
Circulating concentrations of sFlt1 (soluble fins-like tyrosine kinase 1) in fetal and maternal serum during pre-eclampsia
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DOI：
10.1016/j.ejogrb.2004.11.015
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发表时间：
2005-09-01
期刊：
影响因子：
--
通讯作者：
Holthe, MR
中科院分区：
文献类型：
--
作者：
Staff, AC;Braekke, K;Holthe, MR
目的：我们假设子痫前期患者脐静脉血清可溶性fms样酪氨酸激酶1（sFlt1）浓度升高。我们还探讨了胎儿和母体sFlt 1浓度之间的可能关联。研究设计：在剖宫产时，从先兆子痫（n = 38）和无并发症（n = 32）的孕妇血清样本，以及脐静脉血清和羊水样本。采用ELISA法检测sFlt1、血管内皮生长因子（VEGF）和胎盘生长因子（PlGF）。与无并发症妊娠相比，先兆子痫患者脐静脉血清中sFlt1浓度中位数升高在母体血清（9932和3417 pg/mL，P <0.001）以及羊水（51，040和33，490 pg/mL，P = 0.03）中，分别为246和163 pg/mL，P = 0.04。在先兆子痫组中，胎儿和母亲血清sFlt1水平之间呈正相关。先兆子痫组孕妇血清中PIGF浓度中位数显著低于对照组（82 pg/mL和169 pg/mL，P <0.001）。结论：先兆子痫胎儿循环中sFlt1浓度升高，但远低于母体循环中的水平。我们的研究结果不支持胎儿对先兆子痫中母体sFlt1蛋白浓度升高的实质性贡献。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
Objective: We hypothesized that umbilical vein serum soluble fms-like tyrosine kinase 1 (sFlt1) concentration was augmented in pre-eclampsia. We also explored a possible association between fetal and maternal concentrations of sFlt1.Study design: At cesarean delivery, maternal serum samples from pre-eclamptic (n = 38) and uncomplicated (n = 32) pregnancies were obtained, as well as umbilical vein serum and amniotic fluid samples. ELISA for human sFlt1, vascular endothelial growth factor (VEGF) and placental growth factor (PIGF) were performed.Results: Median sFlt1 concentrations were elevated in pre-eclampsia compared to uncomplicated pregnancy, in umbilical venous serum (246 and 163 pg/mL, P = 0.04), in maternal serum (9932 and 3417 pg/mL, P < 0.001), as well as in amniotic fluid (51,040 and 33,490 pg/mL, P = 0.03). A positive association between the fetal and maternal serum levels of sFlt1 was found in the pre-eclampsia group. Median PIGF concentration in the maternal serum was significantly lower in the pre-eclampsia group compared to the control group (82 pg/mL and 169 pg/ mL, P < 0.001).Conclusions: sFlt1 concentration is elevated in the fetal circulation in pre-eclampsia, but at a much lower level than in the maternal circulation. The results of our study do not support a substantial fetal contribution to the elevated circulating maternal sFlt1 protein concentration in pre-eclampsia. (c) 2004 Elsevier Ireland Ltd. All rights reserved.