Craniopharyngioma cell growth is promoted by growth hormone (GH) and is inhibited by tamoxifen: Involvement of growth hormone receptor (GHR) and IGF-1 receptor (IGF-1R)
Craniopharyngioma cell growth is promoted by growth hormone (GH) and is inhibited by tamoxifen: Involvement of growth hormone receptor (GHR) and IGF-1 receptor (IGF-1R)
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生长激素 (GH) 促进颅咽管瘤细胞生长，他莫昔芬抑制细胞生长：生长激素受体 (GHR) 和 IGF-1 受体 (IGF-1R) 的参与
DOI：
10.1016/j.jocn.2012.04.014
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发表时间：
2013-01
影响因子：
2
通讯作者：
Xu, Jianguo
中科院分区：
文献类型：
--
作者：
Rao, Zhengxi;Sima, Xiutian;Zhou, Liangxue;Xu, Jianguo
Hormone receptors are related to the biological behavior and recurrence of craniopharyngioma (CP). The effect of therapeutic growth hormone (GH) replacement on CP growth remains largely unclear. We intended to study expression patterns of GH-related receptors in CP, and to study the effect of GH and its mediator, insulin-like growth factor-1 (IGF-1), as well as tamoxifen, on primary CP cell cultures. Primary cell cultures were established from fresh tumor specimens. The expression of GH receptor (GHR) and IGF-1 receptor (IGF-1R) in tumor specimens was studied using immunohistochemistry. Cell cultures were treated with various concentrations of recombinant GH, IGF-1 and tamoxifen. Cell growth promotion or inhibition was assayed using the Trypan blue dye exclusion test of cell viability. Expression of GHR, IGF-1R, phosphorylated-Akt and Akt after treatment was studied using Western blot assay. Twenty-nine primary cultures from 36 patients were established. GHR and IGF-1R were expressed in tumor tissue. The promotion of cell growth by GH compared to control was most prominent at 100ng/mL, while inhibition by tamoxifen was concentration dependent. IGF-1 was more effective in promoting growth in CP cell cultures with high IGF-1R expression, and it increased phosphorylation of Akt protein. Primary cell cultures can be established in more than 80% of fresh CP specimens. GH and its endogenous mediator, IGF-1, promotes CP cell growth in vitro, while tamoxifen inhibits growth.
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影响因子：
2
作者：
T. Tanaka,;Y. Seino;K. Fujieda;Y. Igarashi;S. Yokoya;K. Tachibana;Y. Ogawa
通讯作者：
T. Tanaka,;Y. Seino;K. Fujieda;Y. Igarashi;S. Yokoya;K. Tachibana;Y. Ogawa
影响因子：
5.8
作者：
Norrman, Lise-Lott;Johannsson, Gudmundur;Svensson, Johan
通讯作者：
Svensson, Johan
影响因子：
2
作者：
Mario Mandalà;Cecilia Moro;Gianluigi Ferretti;M. G. Calabrò;F. Nolè;A. Rocca;E. Munzone;A. Castro;Giuseppe Curigliano
通讯作者：
Mario Mandalà;Cecilia Moro;Gianluigi Ferretti;M. G. Calabrò;F. Nolè;A. Rocca;E. Munzone;A. Castro;Giuseppe Curigliano
DOI：
10.1360/03yc9022
发表时间：
2003-04
期刊：
Science in China Series C: Life Sciences
影响因子：
--
作者：
Qingfu Xu;Zhihui Zhao;Y. Ni;R. Zhao;Jie Chen
通讯作者：
Qingfu Xu;Zhihui Zhao;Y. Ni;R. Zhao;Jie Chen
影响因子：
3.2
作者：
Karavitaki, N;Warner, JT;Wass, JAH
通讯作者：
Wass, JAH