Development of a Series of Fluorescent Probes for the Early Diagnostic Imaging of Sulfur Mustard Poisoning
Development of a Series of Fluorescent Probes for the Early Diagnostic Imaging of Sulfur Mustard Poisoning
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开发用于硫芥中毒早期诊断成像的系列荧光探针
DOI：
10.1021/acssensors.9b01424
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发表时间：
2019-10-01
期刊：
影响因子：
8.9
通讯作者：
Xiao, Kai
中科院分区：
文献类型：
--
作者：
Meng, Wenqi;Sun, Mingxue;Xiao, Kai
芥子气是最有害的化学战剂之一，可引起皮肤、眼睛和肺损伤。但由于芥子气中毒后存在潜伏期，使医务人员很难对芥子气中毒进行早期诊断。体内检测完整的芥子气可能是早期诊断芥子气中毒的有效方法。本研究开发了一系列完整硫芥荧光探针。所研制的探针均能与芥子气发生选择性反应。在这些探针中，SiNIR-SM表现出非常好的响应率和高的关/开对比度。据我们所知，SiNIR-SM是第一个用于硫芥检测的近红外荧光探针。SiNIR-SM和OxSM-1均成功应用于活细胞中硫芥的成像。使用SiNIR-SM，我们发现硫芥积累在活细胞的线粒体中。这一结果可能为硫芥损伤的治疗提供新的见解。我们还发现SiNIR-SM适合通过检测完整的硫芥来对SKH-1小鼠硫芥中毒进行早期诊断成像。
Sulfur mustard is one of the most harmful chemical warfare agents and can induce skin, eye, and lung injuries. However, it is hard for medical stuff to diagnose sulfur mustard poisoning early because of the incubation period after sulfur mustard exposure. Detecting intact sulfur mustard in vivo might be an effective approach for the early diagnosis of sulfur mustard poisoning. A series of fluorescent probes for intact sulfur mustard detection were developed in this study. All of the developed probes could react with sulfur mustard selectivity. Among these probes, SiNIR-SM exhibited an extremely good response rate and a high off/on contrast. To the best of our knowledge, SiNIR-SM is the first near-infrared fluorescent probe for the sulfur mustard detection. Both SiNIR-SM and OxSM-1 were successfully applied to image sulfur mustard in living cells. Using SiNIR-SM, we found that sulfur mustard accumulates in the mitochondria of living cells. This result could provide a new insight for the treatment of sulfur mustard injuries. We also found that SiNIR-SM is suitable for the early diagnostic imaging of sulfur mustard poisoning in SKH-1 mice via the detection of intact sulfur mustard.